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In vitro and in vivo correlation of skin and cellular responses to nucleic acid deliveryOmerzel, Maša, mag. farm. ...Skin, the largest organ in the body, provides a passive physical barrier against infection and contains elements of the innate and adaptive immune systems. Skin consists of various cells, including ... keratinocytes, fibroblasts, endothelial cells and immune cells. This diversity of cell types could be important to gene therapies because DNA transfection could elicit different responses in different cell types. Previously, we observed the upregulation and activation of cytosolic DNA sensing pathways in several non-tumor and tumor cell types as well in tumors after the electroporation (electrotransfer) of plasmid DNA (pDNA). Based on this research and the innate immuno- genicity of skin, we correlated the effects of pDNA electrotransfer to fibroblasts and keratinocytes to mouse skin using reverse transcription real-time PCR (RT-qPCR) and several types of protein quantification. After pDNA electrotransfer, the mRNAs of the putative DNA sensors DEAD (AspGlu-Ala-Asp) box polypeptide 60 (Ddx60), absent in melanoma 2 (Aim2), Z-DNA binding protein 1 (Zbp1), interferon activated gene 202 (Ifi202), and interferon-inducible protein 204 (Ifi204) were upregulated in keratinocytes, while Ddx60, Zbp1 and Ifi204 were upregulated in fibroblasts. Increased levels of the mRNAs and proteins of several cytokines and chemokines were detected and varied based on cell type. Mouse skin experiments in vivo confirmed our in vitro results with increased expression of putative DNA sensor mRNAs and of the mRNAs and proteins of several cytokines and chemokines.Source: Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. - ISSN 0753-3322 (Vol. 150, Jun. 2022, str. 113088-1-113088-13)Type of material - article, component partPublish date - 2022Language - englishCOBISS.SI-ID - 107308035
Author
Omerzel, Maša, mag. farm. |
Žnidar, Katarina |
Sales Conniff, A. |
Jesenko, Tanja |
Markelc, Boštjan |
Tur, Jared |
Semenova, Nina |
Kohena, Kristopher |
Kranjc Brezar, Simona |
Heller, Loree C. |
Čemažar, Maja
Topics
senzorji DNK |
citokini |
elektroprenos |
koža |
DNA sensors |
cytokines |
electrotransfer |
skin
Author | Omerzel, Maša, mag. farm. ... |
Title | In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery |
Publication date | 2022-05-10 |
COBISS.SI-ID | 107308035 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev | P3-0003-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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https://www.sciencedirect.com/science/article/pii/S0753332222004772 |
https://dirros.openscience.si/IzpisGradiva.php?id=15448 |
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APC – Article Processing Charge | 2342.40 € |
2342.40 € : Onkološki inštitut Ljubljana |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Omerzel, Maša, mag. farm. | 34373 |
Žnidar, Katarina | 37534 |
Sales Conniff, A. | |
Jesenko, Tanja | 33227 |
Markelc, Boštjan | 32175 |
Tur, Jared | |
Semenova, Nina | |
Kohena, Kristopher | |
Kranjc Brezar, Simona | 19058 |
Heller, Loree C. | |
Čemažar, Maja | 14575 |
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