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Critical steps and troubleshooting in sample preparation for wood and phloem formation : from sampling to microscopic observation = Kritični koraki in reševanje težav pri pripravi vzorcev za spremljanje nastajanja lesa in floema : od vzorčenja do opazovanja pod mikroskopomBalzano, Angela ...We present a technical note that supplements published procedures on optimal sample preparation for performing wood and phloem formation analyses. Before beginning sampling, it is important to learn ... about the characteristics of the tree or shrub species to be investigated. Some tips are given how to use the Trephor tool in the best way, how to remove the outer hard bark (periderm), how microcores should be handled after removal from the tree, and how they should be oriented for embedding in paraffin, and cutting thin sections for microscopy. Possible defects that may result from improper handling are illustrated and discussed. We also present optimal images to accurately identify different cell development stages in phloem and xylem, which is particularly challenging in hardwoods and Mediterranean tree and shrub species.Source: Les = Wood. - ISSN 0024-1067 (Letn. 71, št. 1, 2022, str. 47-56)Type of material - article, component part ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 116719619
Digital content: dCOBISS
| Author | Balzano, Angela ... |
| Title | Critical steps and troubleshooting in sample preparation for wood and phloem formation : from sampling to microscopic observation = Kritični koraki in reševanje težav pri pripravi vzorcev za spremljanje nastajanja lesa in floema : od vzorčenja do opazovanja pod mikroskopom |
| Publication date | 2022-06-30 |
| COBISS.SI-ID | 116719619 |
| Publication version in repository | Publisher's version |
| Publication licence | Creative Commons Attribution-NonCommercial 4.0 International |
| Embargo | Immediate publication for public |
Project(s) from which the publication was funded
| Title | Acronym | Project ID | Funder |
|---|---|---|---|
| Les in lignocelulozni kompoziti | P4-0015-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
https://www.les-wood.si/index.php/leswood/article/view/160/110
