-
Kin discrimination modifies strain distribution, spatial segregation and incorporation of extracellular matrix polysaccharide mutants of Bacillus subtilis strains into mixed floating biofilms [Elektronski vir]Bolješić, Maja ...Microorganisms in nature form multicellular groups called biofilms. In biofilms, bacteria embedded in the extracellular matrix (ECM) interact intensely due to their proximity. Most studies have ... investigated genetically homogeneous biofilms, leaving a gap in knowledge on genetically heterogeneous biofilms. Recent insights show that a Gram-positive model bacterium, Bacillus subtilis, discriminates between strains of high (kin) and low (nonkin) genetic similarity, reflected in merging (kin) and boundaries (nonkin) between swarms. However, it is unclear how kinship between interacting strains affects their fitness, the genotype assortment, and incorporation of the mutant lacking the main structural ECM polysaccharide (EpsA-O) into floating biofilms (pellicles). We cultivate Bacillus subtilis strains as mixtures of isogenic, kin and nonkin strain combinations in the biofilm-promoting medium (MSgg) in static conditions, allowing them to form pellicles. We show that in nonkin pellicles, the dominant strain strongly reduced the frequency of the other strain. Segregation of nonkin mixtures in pellicles increased and invasion of nonkin EpsA-O-deficient mutants into pellicles decreased compared to kin and isogenic floating biofilms. Kin and isogenic strains had comparable relative frequencies in pellicles and showed more homogenous cell mixing. Overall, our results emphasize kin discrimination as a social behavior that shapes strain distribution, spatial segregation and ECM mutant ability to incorporate into genetically heterogenous biofilms of B. subtilisSource: Applied and environmental microbiology [Elektronski vir]. - ISSN 1098-5336 (Vol. 88, iss. 18, Sep. 2022, str. [1-14])Type of material - e-article ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 117918979
Author
Bolješić, Maja |
Kraigher, Barbara, mikrobna ekologinja |
Dogša, Iztok |
Jerič Kokelj, Barbara, 1987- |
Mandić-Mulec, Ines
Topics
mikrobne združbe |
biofilm |
socialne interakcije |
sorodstvena diskriminacija |
zunajcelični polisaharidi |
Bacillus subtilis |
biofilms |
kin discrimination |
incorporation of extracellular matrix polysaccharide mutant
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Author | Bolješić, Maja ... |
Title | Kin discrimination modifies strain distribution, spatial segregation and incorporation of extracellular matrix polysaccharide mutants of Bacillus subtilis strains into mixed floating biofilms [Elektronski vir] |
Publication date | 2022-12-09 |
COBISS.SI-ID | 117918979 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Mikrobiologija in biotehnologija živil in okolja | P4-0116-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Raziskave medceličnih komunikacij v večceličnih skupnostih različnih izolatov bakterije iz rodu Bacillus | J4-9302-2018 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Razvoj mikropretočnih sistemov za analizo, izbor in uporabo bakterijskih celic | J4-1775-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Platforma osnovana na sintetičnih biofilmih za preučevanje in razvoj novih proti biofilmskih pristopov | J1-3021-2021 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Chair Of Micro Process Engineering and TEchnology | COMPETE | 811040 |
European Commission |
Files that belong to the publication
Link |
---|
![]() |
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Bolješić, Maja | 50600 |
Kraigher, Barbara, mikrobna ekologinja | 22492 |
Dogša, Iztok | 24407 |
Jerič Kokelj, Barbara, 1987- | 33349 |
Mandić-Mulec, Ines | 05993 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.