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Accuracy of Allplex SARS-CoV-2 assay amplification curve analysis for the detection of SARS-CoV-2 variant AlphaŽohar Čretnik, Tjaša ...Aim: To evaluate the accuracy of two PCR-based techniques for detecting SARS-CoV-2 variant Alpha (B.1.1.7). Materials & methods: A multicenter prospective cohort with 1137 positive specimens from ... Slovenia was studied. A mutation-based assay (rTEST-COVID-19 qPCR B.1.1.7 assay) and amplification curve pattern analysis of the Allplex SARS-CoV-2 assay were compared with whole-genome sequencing. Results: SARS-CoV-2 variant Alpha was detected in 155 samples (13.6%). Sensitivity and specificity were 98.1 and 98.0%, respectively, for the rTEST-COVID-19 qPCR B.1.1.7 assay and 97.4 and 97.5%, respectively, for amplification curve pattern analysis. Conclusion: The good analytical performance of both methods was confirmed for the preliminary identification of SARS-CoV-2 variant Alpha. This cost-effective principle for screening SARS-CoV-2 populations is also applicable to other emerging variants and may help to conserve some whole-genome sequencing resources.Source: Future microbiology. - ISSN 1746-0913 (Vol. 17, no. 4, 2022, str. 1125–1131)Type of material - article, component partPublish date - 2022Language - englishCOBISS.SI-ID - 120530691
Author
Žohar Čretnik, Tjaša |
Retelj, Matjaž |
Janežič, Sandra, mikrobiologinja |
Mahnič, Aleksander |
Tesovnik, Tine |
Šket, Robert, 1988- |
Jenko Bizjan, Barbara, 1988- |
Jeverica, Samo |
Ravnik, Mateja, 1969- |
Rak, Mitja |
Borinc, Mateja, mikrobiologija, 1975- |
Rupnik, Maja, 1967- |
Battelino, Tadej |
Pokorn, Marko |
Kovač, Jernej, 1983-
Topics
B.1.1.7 |
SARS-CoV-2 |
Seegene SARS-CoV-2 assay |
Slovenia |
variant of concern
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Žohar Čretnik, Tjaša | 20741 |
Retelj, Matjaž | ![]() |
Janežič, Sandra, mikrobiologinja | 30755 |
Mahnič, Aleksander | 37544 |
Tesovnik, Tine | 37490 |
Šket, Robert, 1988- | 37426 |
Jenko Bizjan, Barbara, 1988- | 35356 |
Jeverica, Samo | 25987 |
Ravnik, Mateja, 1969- | 15398 |
Rak, Mitja | 32084 |
Borinc, Mateja, mikrobiologija, 1975- | ![]() |
Rupnik, Maja, 1967- | 12278 |
Battelino, Tadej | 13023 |
Pokorn, Marko | 15476 |
Kovač, Jernej, 1983- | 32181 |
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