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Sposobnost biokonverzije ricinovega olja v laktone pri kvasovkah : diplomska naloga = The yeasts capacity for bioconversion of castor oil into lactones : graduation thesis
Jejčič, Bruna
Namen diplomske naloge je ugotoviti rast testiranih kvasovk v sestavljenem gojišču z dodanim ricinovim oljem kot laktonskim prekurzorjem ter sposobnost biokonverzije prekurzorja v laktone. Biomaso ...smo submerzno namnoževali na rotacijskem stresalniku pod aerobnimi razmerami v sestavljenem gojišču in v sestavljenem gojišču z 2 odstotka ricinovega olja. Rast kvasovk smo spremljaliz merjenjem pH vrednosti in ▫$pO_2$▫. Na nobeno od testiranih kvasovk ricinovo olje ni delovalo inhibitorno. Laktone smo ekstrahirali s selektivno ekstrakcijo in analizirali s plinsko kromatografijo z metodo eksternega standarda. Laktone, ki smo jih analizirali, so: ▫$\gamma$▫-oktalakton, ▫$\delta$▫-dekalakton, ▫$\delta$▫-dekalakton, ▫$\delta$▫-undekalakton in ▫$\delta$▫-dodekalakton. Kvasovka ▫{\sl Candida lipolytica}▫ ZIM 14 je proizvedla 38,57 mg/l skupnih laktonov, ▫{\sl Yarrovia lipolytica}▫ ZIM 39 43,19 mg/l, ▫{\sl Hansenula anomala}▫ ZIM 47 3,23 mg/l, ▫{\sl Pichia ohmeri}▫ ZIM 42 2,03 mg/l, ▫{\sl Kluyveromyces marxianus}▫ ZIM 73 1,65 mg/l, ▫{\sl Sporobolomyces gracilis}▫ ZIM 54 1,28 mg/l, ▫{\sl Kluyveromyces marxianus}▫ ZIM 1 1,17 mg/l, ▫{\sl Rhodotorula aurantiaca}▫ ZIM 53 1,05 mg/l, ▫{\sl Sporobolomyces pollaccii}▫ ZIM 10 0,22 mg/l in ▫{\sl Saccharomyces cerevisiae}▫ ZIM 410 0,14 mg/l.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [B. Jejčič], 1996
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