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Določanje ekstracelularnega in intracelularnega cistatina C v tumorskih celičnih linijah = Determination of extracellular and intracellular cystatin C in tumor cell lines : diplomska naloga
Avanzo, Matej, 1978
Cistatin C je inhibitor cisteinskih proteaz, ki imajo pomembno vlogo pri invaziji in metastaziranju tumorskih celic.V tem diplomskem delu smo doloèali koncentracije cistatina C v stirih razlièno ...invazivnih celiènih linijah Hep-2C, U-937, MCF-10A neoT in MDA-MB-231 z namenom, da ugotovimo povezanost njegove koncentracije s stopnjo invazivnosti celiène linije. Cistatin C smo kvantitativno doloèili v intracelularnih in ekstracelularnih frakcijah z direktnim ELISA testom, nismo pa ga uspeli doloèiti z imunskim pivnanjem ali merjenjem njegove inhibitorne aktivnosti. Ugotovili smo, da je celokupna koncentracija cistatina C v invazivnih celiènih linijah MCF-10A neoT in MDA-MB-231 nekoliko visja kot pri neinvazivnih celiènih linijah, bistvene pa so razlike med intracelularnimi in ekstracelularnimi frakcijami. Pri invazivnih celicah je ekstracelularnega cistatina mnogo veè kakor intracelularnega, v nasprotju z neinvazivnimi celicami, kjer je ekstracelularnega cistatina manj kakor intracelularnega.
Type of material - undergraduate thesis
Publication and manufacture - Ljubljana : [Avanzo M.], 2003
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