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A novel approach of direct ex vivo epitope mapping identifies dominant and subdominant CD4 and CD8 T cell epitopes from Listeria monocytogenesGeginat, Gernot ...We used a novel approach for the direct ex vivo identification and characterization of T cell epitopes based on the screening of peptide spot libraries with freshly isolated splenocytes in a ... sensitive enzyme-linked immunospot (ELISPOT) assay. This technique was applied for the analysis of splenocytes from Listeria monocytogenes-infected BALB/c and C57BL/6 mice. The screening of peptide spot libraries covering the whole listeriolysin O and p60of L. monocytogenes confirmed all known CD4 and CD8 T cell epitopes of these proteins and additionally revealed six new H-2(d) and six new H-2(b)-restricted T cell epitopes. New epitopes were categorized into CD4 and CD8 T cell epitopes by ex vivo ELISPOT analysis with separated T cell populations.The quantitative analysis of cells reactive with these CD4 and CD8T cell epitopes revealed the existence of dominant and subdominant CD4 and CD8 T cell populations during L. monocytogenes infection. As a consequence of these data we suggest that ELISPOT-based screening of peptide spot libraries could be a general approach for the rapid identification and characterization of pathogen-specific T cell populations during various infectious diseases.Source: The journal of immunology. - ISSN 0022-1767 (Letn. 166, št. 3, 2001, str. 1877-1884)Type of material - article, component partPublish date - 2001Language - englishCOBISS.SI-ID - 14371801
Author
Geginat, Gernot |
Schenk, Simone |
Škoberne, Mojca, 1972- |
Goebel, Werner |
Hof, Herbert
Topics
Listeria monocytogenes |
Immunology |
Epitope mapping |
Epitopes, t-lymphocyte |
Analysis |
Immunology |
Cd4-positive t-lymphocytes |
Immunology |
Microbiology |
Metabolism |
Cd8-positive t-lymphocytes |
Immunology |
Microbiology |
Metabolism |
Mice, inbred balb c |
Mice, inbred c57bl |
T-lymphocyte subsets |
Peptide library |
Lymphocyte transformation |
Immunophenotyping |
Immunologic memory |
Histocompatibility antigens class i |
Histocompatibility antigens class ii |
Heat-shock proteins |
Cell line |
Enzyme-linked immunosorbent assay |
Bacterial proteins |
Immunology |
Analysis |
Epitop, mapiranje |
Epitopi |
Limfociti T |
Listeria monocytogenes |
Bakterijske beljakovine |
Celična linija |
ELISA |
Histokompatibilnost, antigeni |
Imunofenotipizacija |
Imunski spomin |
Limfocit, transformacija |
Limfocitne podenote |
Miši seva BALB C |
Miši seva C57BL |
Peptidna knjižnica |
Vročinski-šok beljakovine
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Škoberne, Mojca, 1972- | 18356 |
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