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Histolocalisation and computer aided reconstruction of polyphenol distributionin seeds
Kreft, Marko ; Kreft, Samo
Computer aided three-dimensional reconstruction was used to analyze the two dimensional images obtained by progressively grinding of buckwheat seeds. The seeds were embedded into polyester resin and ...100 micro m thick slices were progressively removed. Each newly exposed plane was treated with the FeCl3 to produce a dark blue color with polyphenols and photographed. The photographs were imported into Silicon graphics computer and three-dimensional picture of the distribution of polyphenols was reconstructed. On the three dimensional reconstruction of the seed the distribution of polyphenols can be observed. The method is feasible to be applied to study the distribution of polyphenols as well in other seeds or plant parts with a complex structure.
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