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Vpliv ergotamina na rast kvasovk : diplomska naloga = The influence of ergotamine on the growth of yeasts : graduation thesis
Zagorc, Tatjana, 1970-
Namen naloge je bil proučevanje vpliva ergotamina na rast kvasovk. Ergotamin spada v skupino ergot alkaloidov, ki so sekundarni produkti matabolizma višjih gliv iz rodu ▫{\sl Claviceps}▫. ...Eksperimentalno delo je bilo razdeljenov tri sklope. Namen kultivacije na različnih trdih gojiščih je bil zožiti izbor kvasovk glede na njihovo občutljivost na ergotamin. Sledil je sklop kultivacij v tekočih gojiščih z in brez dodatka ergotamina. V ta sklop so bile vključene kvasovke i7, i9, ZIM 419, ki so izolirane iz odpadne brozge in kvasovka ZIM 3 iz Zbirke industrijskih mikroorganozmov, ki je služila za primerjavo. Proučevanje vpliva ergotamina na rast kvasovk vključuje sočasno spremljanje rastnih parametrov pH vrednosti in ▫$pO_2$▫ ter meritve masnih koncentracij ergotamina z metodo po van Urku in množine biomase. Rezultati kažejo, da dodatek ergotamina nima inhibitornega vpliva na rast kvasovk v tekočem gojišču. Spremembe koncentracij ergotamina so posledica kosmičenja in vezave na biomaso. Tretji sklop predstavlja kultivacija v bioreaktorju s sočasnimi merjanji pH vrednosti in ▫$pO_2$▫. Koncentracije ergotamina v procesni brozgi so bile določene z metodo po van Urku in s HPLC. Zaznana je bila sprememba koncentacije ergotamina, kar nakazuje možnost njegove biološke razgradnje.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [T. Zagorc], 1995
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