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  • Emulsion templated open porous membranes for protein purification
    Pulko, Irena, 1980- ...
    Approximately 25 cm * 25 cm large sheets of crosslinked highly porous poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate-co-ethylhexyl methacrylate) membranes with an average thicknesses ... between 285 and 565 m were prepared by casting a high internal phase emulsion (HIPE) containing monomers onto glass substrates and subsequent polymerisation. Open cellular porous polyHIPE type membranes were obtained with large pores (cavity) sizes between 3 and 10 m while interconnecting pores were between 1 and 3 m. The percentage of ethylhexyl acrylate and ethyleneglycol dimethacrylate influenced the flexibility and morphology of the resulting membranes. Porous membranes were chemically modified with diethylamine to yield functionalised supports for ion exchange chromatography. Cylindrical housings were used for positioning of the membranes and allowing flow of the mobile phase. Pulse experiments were used to study the flow characteristics and a homogeneous flowthrough the entire area of the membrane was found. Bovine serum albumin was purified by a 8 ml column containing functional membrane in modular shape;dynamic binding capacity was measured to be as high as 45 mg/ml.
    Source: Selected Papers of the 4th Summer School of Monolith Technology for Biochromatography, Bioconversion and Phase State Synthesis, Portorož, Slovenia, 30 May-02 June 2010 (Vol. 1218, iss. 17, 2011, str. 2396-2401)
    Type of material - conference contribution
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 14668054
    DOI

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