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Overexpression of Raidd cDNA inhibits differentiation of mouse preadipocytesFemer, R. ...RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. To study the possibility of a novel ... system of cell ablation mediated by RAIDD, a predipocyte cell line (3T3L1) was stably transfected with a plasmid contaning the murine Raidd cDNA under the control of the adipocyte cell line (3T3L1) was stably transfected with a plasmid contaning the murine Raidd cDNA under the control of the adipocyte specific promoter aP2. Instead of the expected apoptosis, a blockage to differentiation upon hormonal induction was observed as judged by an absence of lipid accumulation, a lack of expression of adipocyte-specific genes and a fibroblastic appearance. Proliferation rate of Raidd-transfected clones remained unaffected. Overexpression of Raidd cDNA in 3T3L1 cell therefore inhibited differentiation, suggesting that Raidd plays a role in controlling differentiation of mouse preadipocytes and, perhaps, in other cell types, in addition to its established role in apoptosis.Source: Cell proliferation. - ISSN 0960-7722 (Letn. 36, št. 1, 2003, str. 45-54)Type of material - article, component partPublish date - 2003Language - englishCOBISS.SI-ID - 1502344
Author
Femer, R. |
Horvat, Simon |
Clinton, Michael |
Clark, A.J.
Topics
molekularna genetika |
geni |
izražanje |
Raidd |
cDNK |
adipociti |
apoptoze |
miši
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Database name | Field | Year |
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Femer, R. | ![]() |
Horvat, Simon | 10412 |
Clinton, Michael | ![]() |
Clark, A.J. | ![]() |
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