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Enzymatic digestion of partially and fully regenerated cellulose model films from trimethylsilyl celluloseMohan, Tamilselvan ...Partially and fully regenerated cellulose model films from trimethylsilyl cellulose (TMSC) were prepared by a time dependent regeneration approach. These thin films were characterized with contact ... angle measurements and attenuated total reflectance infrared spectroscopy (ATR-IR). In order to get further insights into the completeness of the regeneration we studied the interaction of cellulase enzymes from Trichoderma viride with the cellulose films using a quartz crystal microbalance with dissipation (QCM-D). To supportthe results from the QCM-D experiments capillary zone electrophoresis (CZE) and atomic force microscopy (AFM) were applied. The changes in mass and energy dissipation due to the interaction of the enzymes with the substrates were correlated with the surface wettability and elemental composition of the regenerated films. The highest interaction activity between the films and the enzyme, as well as the highest cellulose degradation, was observed on fully regenerated cellulose films, but some degradation also occurred on pure TMSC films. The enzymatic degradation rate correlated well with the rate of regeneration. It was demonstrated that CZE can be used to support QCM-D data via the detection of enzyme hydrolysis products in the eluates of the QCM-D cells. Glucose release peaked at the same time as the maximum mass loss was detected via QCM-D. It was shown that a combination of QCM-D and CZE together with enzymatic digestion is a reliable method to determine the conversion rate of TMSC to cellulose. In addition QCM-D and AFM revealed that cellulase is irreversibly bound to hydrophobic TMSC surfaces, while pure cellulose is digested almost completely in the course of hydrolysis.Source: Polysaccharides as source of advanced and sustainable products (Str. 191-198)Type of material - conference contributionPublish date - 2013Language - englishCOBISS.SI-ID - 15804182
Author
Mohan, Tamilselvan |
Kargl, Rupert |
Doliška, Aleš |
Ehmann, Heike M. A. |
Ribitsch, Volker |
Stana-Kleinschek, Karin
Topics
celuloza |
celulozni modelni filmi |
kremenova mikrotehtnica |
kapilarna conska elektroforeza |
omočljivost |
ATR infrardeča spektroskopija |
cellulase |
cellulose model films |
quartz crystal microbalance |
capillary zone electrophoresis |
wettability |
attenuated total reflectance infrared spectroscopy
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Database name | Field | Year |
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Mohan, Tamilselvan | 33768 |
Kargl, Rupert | 36303 |
Doliška, Aleš | 17577 |
Ehmann, Heike M. A. | 33769 |
Ribitsch, Volker | 15077 |
Stana-Kleinschek, Karin | 07814 |
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