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Analiza genetske strukture primorske kozje češnje (Rhamnus intermedius Steud. & Hochst.) v Sloveniji [Elektronski vir] : magistrsko delo - 2. stopnja = Analysis of genetic structure of Rhamnus intermedius Steud. & Hochst. in Slovenia : M. Sc. Thesis - master study programmesLišter, MonikaPrimorska kozja češnja (Rhamnus intermedius Steud. & Hochst.) je pred kratkim postala nova predstavnica slovenske flore. Na zaraščajočem travniku Kraškega roba je bil opažen en sam grm, sestavljen iz ... več morfološko različnih poganjkov. Prvič je bila izvedena izolacija DNA iz slovenske populacije primorske kozje češnje. Poleg tega je bilo na tej vrsti prvič preizkušano delovanje mikrosatelitskih markerjev, ki so bili že prej uporabljeni na drugih sorodnih vrstah iz rodu Rhamnus. V raziskavo sta bili vključeni slovenska in hrvaška populacija, kot referenčni vrsti pa sta služili razkrečena (R. saxatilis) in kranjska kozja češnja (R. fallax). Analiza sedmih mikrosatelitskih lokusov je v slovenskem grmu primorske kozje češnje določila šest različnih genotipov. Populacija je, v nasprotju s hrvaško, ki povprečno izkazuje pomanjkanje heterozigotov (F = 0,281), precej variabilna (F = -0,204). V vzorcu hrvaške populacije je bil na treh lokusih potrjen odmik od Hardy-Weinbergovega ravnovesja. Med populacijama se kaže zmerna genetska diferenciacija (FST = 0,088). Primerjava genetske slike treh vrst kozje češnje je bila izvedena z analizo glavnih koordinat. Iz analize je razvidno, da opazovani grm pripada vrsti Rhamnus intermedius. Rezultati raziskave potrjujejo, da ima populacija možnosti za medsebojno opraševanje in razmnoževanje s semenom.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [M. Lišter], 2023Language - slovenianCOBISS.SI-ID - 158979331
Author
Lišter, Monika
Other authors
Jarni, Kristjan
Topics
mikrosatelitski markerji |
genetska pestrost |
populacija |
primorska kozja češnja |
Slovenija |
microsatellite markers |
genetic diversity |
population |
Rhamnus intermedius |
Slovenia
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Lišter, Monika | |
Jarni, Kristjan | 26070 |
Source: Personal bibliographies
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