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The effect of forskolin and the role of Epac2A during activation, activity, and deactivation of beta cell networks [Elektronski vir]Skelin, Maša ...Beta cells couple stimulation by glucose with insulin secretion and impairments in this coupling play a central role in diabetes mellitus. Cyclic adenosine monophosphate (cAMP) amplifies ... stimulus-secretion coupling via protein kinase A and guanine nucleotide exchange protein 2 (Epac2A). With the present research, we aimed to clarify the influence of cAMP-elevating diterpene forskolin on cytoplasmic calcium dynamics and intercellular network activity, which are two of the crucial elements of normal beta cell stimulus-secretion coupling, and the role of Epac2A under normal and stimulated conditions. To this end, we performed functional multicellular calcium imaging of beta cells in mouse pancreas tissue slices after stimulation with glucose and forskolin in wild-type and Epac2A knock-out mice. Forskolin evoked calcium signals in otherwise substimulatory glucose and beta cells from Epac2A knock-out mice displayed a faster activation. During the plateau phase, beta cells from Epac2A knock-out mice displayed a slightly higher active time in response to glucose compared with wild-type littermates, and stimulation with forskolin increased the active time via an increase in oscillation frequency and a decrease in oscillation duration in both Epac2A knock-out and wild-type mice. Functional network properties during stimulation with glucose did not differ in Epac2A knock-out mice, but the presence of Epac2A was crucial for the protective effect of stimulation with forskolin in preventing a decline in beta cell functional connectivity with time. Finally, stimulation with forskolin prolonged beta cell activity during deactivation, especially in Epac2A knock-out mice.Source: Frontiers in endocrinology [Elektronski vir]. - ISSN 1664-2392 (Vol. 14, Avg. 2023, str. 1-14)Type of material - e-articlePublish date - 2023Language - englishCOBISS.SI-ID - 163143427
Author
Skelin, Maša |
Dolenšek, Jurij |
Križančić Bombek, Lidija |
Pohorec, Viljem |
Gosak, Marko, 1982- |
Rupnik, Marjan |
Stožer, Andraž
Topics
pancreas |
tissue slices |
beta cells |
calcium imaging |
amplifying pathway |
forskolin |
Epac2A KO |
intercellular network
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Author | Skelin, Maša ... |
Title | The effect of forskolin and the role of Epac2A during activation, activity, and deactivation of beta cell networks [Elektronski vir] |
Publication date | 2023-08-28 |
COBISS.SI-ID | 163143427 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Celične in tkivne mreže | P3-0396-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Files that belong to the publication
Costs of publication
Cost type | Total cost | Payments |
---|---|---|
APC – Article Processing Charge | 3575.67 € |
3575.67 € : Univerza v Mariboru, Medicinska fakulteta |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Skelin, Maša | 29565 |
Dolenšek, Jurij | 23415 |
Križančić Bombek, Lidija | 24423 |
Pohorec, Viljem | 39524 |
Gosak, Marko, 1982- | 28405 |
Rupnik, Marjan | 12266 |
Stožer, Andraž | 32132 |
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