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Expression of mesenchymal stem cells-related genes and plasticity of aspirated follicular cells obtained from infertile women [Elektronski vir]Džafić, Edo ...After removal of oocytes for in vitro fertilization, follicular aspirates which are rich in somatic follicular cells are discarded in daily medical practice. However, there is some evidence that less ... differentiated cells with stem cell characteristics are present among aspirated follicular cells (AFCs). The aim of this study was to culture AFCs in vitro and to analyze their gene expression profile. Using the RT2 Profiler PCR array, we investigated the expression profile of 84 genes related to stemness, mesenchymal stem cells (MCSs), and cell differentiation in AFCs enriched by hypoosmotic protocol from follicular aspirates of infertile women involved in assisted reproduction programme in comparison with bone marrow-derived mesenchymal stem cells (BM-MSCs) and fibroblasts. Altogether the expression of 57 genes was detected in AFCs: 16 genes (OCT4, CD49f, CD106, CD146, CD45, CD54, IL10, IL1B, TNF, VEGF, VWF, HDAC1, MITF, RUNX2, PPARG, and PCAF) were upregulated and 20 genes (FGF2, CASP3, CD105, CD13, CD340, CD73, CD90, KDR, PDGFRB, BDNF, COL1A1, IL6, MMP2, NES, NUDT6, BMP6, SMURF2, BMP4, GDF5, and JAG1) were downregulated in AFCs when compared with BM-MSCs. The genes which were upregulated in AFCs were mostly related to MSCs and connected with ovarian function, and differed from those in fibroblasts. The cultured AFCs with predominating granulosa cells were successfully in vitro differentiated into adipogenic-, osteogenic-, and pancreatic-like cells. The upregulation of some MSC-specific genes and in vitro differentiation into other types of cells indicated a subpopulation of AFCs with specific stemness, which was not similar to those of BM-MSCs or fibroblasts.Source: BioMed research international [Elektronski vir]. - ISSN 2314-6141 (Vol. 2014, 2014)Type of material - e-articlePublish date - 2014Language - englishCOBISS.SI-ID - 1727099
Author
Džafić, Edo |
Štimpfel, Martin |
Novaković, Srdjan |
Škerl, Petra, doktorica biologije |
Virant-Klun, Irma
Topics
jajčne celice |
geni |
genetika |
oocytes |
genes |
gene profile
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Džafić, Edo | ![]() |
Štimpfel, Martin | 33917 |
Novaković, Srdjan | 08007 |
Škerl, Petra, doktorica biologije | 28388 |
Virant-Klun, Irma | 06087 |
Source: Personal bibliographies
and: SICRIS
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