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First report on Leptospira species isolated from patients in Slovenia [Elektronski vir]Ružić-Sabljić, Eva ...Leptospirosis is an important worldwide zoonosis, and it has also been reported in Slovenia. The cultivation of Leptospira from human material is difficult. Despite that, we successfully isolated 12 ... human Leptospira strains isolated from patients between 2002 and 2020 and used various methods for the phenotypic and genotypic characterization of the strains, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) using our own MALDI-TOF data library, melting temperature analysis of the amplified lfb1 gene, determination of Leptospira serogroups using rabbit immune sera, NotI-RFLP of the whole Leptospira genome, multilocus sequence typing (MLST) of seven housekeeping genes, and whole-genome sequencing (WGS)-based typing. We confirmed the presence of four pathogenic Leptospira species (L. kirschneri, L. interrogans, L. borgpetersenii, and L. santarosai) and three serogroups: Grippotyphosa, Icterohaemorrhagiae, and Sejroe. MALDI-TOF identified three of seven isolates at the species level and four isolates at the genus level. Serovars of 8 of the 10 strains were determined using NotI-RFLP. MLST showed that the clinical isolates belonged to sequence types ST17, ST110, and ST155. WGS confirmed the analysis of Leptospira strains using conventional methods. In addition, WGS provided better taxonomic resolution for isolate DDA 10944/10.Source: Microorganisms [Elektronski vir]. - ISSN 2076-2607 (Vol. 11, iss. 11, [article no.] 2739, 2023, str. 1-15)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 172934403
Author
Ružić-Sabljić, Eva |
Podgoršek, Daša |
Strašek, Katja |
Celar Šturm, Andraž |
Logar, Mateja, 1970- |
Pavlović, Andrea |
Remec-Zafred, Tatjana |
Baklan, Zvonko |
Pal, Emil |
Cerar Kišek, Tjaša, 1980-
Topics
Leptospira sp. |
leptospirosis |
Slovenia |
1fb gene |
MALDI-TOF |
NotI-RFLP |
MLST |
WGS |
Leptospira |
leptospiroza |
Slovenija |
gen 1fb
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Ružić-Sabljić, Eva | 13296 |
Podgoršek, Daša | |
Strašek, Katja | 25989 |
Celar Šturm, Andraž | |
Logar, Mateja, 1970- | 16183 |
Pavlović, Andrea | |
Remec-Zafred, Tatjana | |
Baklan, Zvonko | 20402 |
Pal, Emil | 34071 |
Cerar Kišek, Tjaša, 1980- | 24350 |
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