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Simple HPLC-UV method for therapeutic drug monitoring of 12 antiepileptic drugs and their main metabolites in human plasma [Elektronski vir]Milosheska, Daniela, farmacevtka ; Roškar, Robert, 1975-The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic ... drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl–Hexyl column at 40 °C, using methanol and potassium phosphate buffer (25 mM; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1–50 mg/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide; 0.5–50 mg/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine; 0.2–10 mg/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide; 0.1–10 mg/L for oxcarbazepine; 2–100 mg/L for felbamate and 3–150 mg/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy.Source: Molecules [Elektronski vir]. - ISSN 1420-3049 (Vol. 28, iss. 23, [article no.] 7830, 2023, 15 str.)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 174900739
Author
Milosheska, Daniela, farmacevtka |
Roškar, Robert, 1975-
Topics
Tekočinska kromatografija |
Epilepsija |
antiepileptična zdravila |
človeška plazma |
terapevtsko spremljanje zdravil |
validacija bioanalitskih metod |
tekočinska kromatografija visoke ločljivosti |
antiepileptic drugs |
human plasma |
therapeutic drug monitoring |
bioanalytical method validation |
high-performance liquid chromatography
Author | Milosheska, Daniela, farmacevtka ; Roškar, Robert, 1975- |
Title | Simple HPLC-UV method for therapeutic drug monitoring of 12 antiepileptic drugs and their main metabolites in human plasma [Elektronski vir] |
Publication date | 2023-11-28 |
COBISS.SI-ID | 174900739 |
Publication version in repository | Publisher's version |
Publication licence | Unidentified |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih | P1-0189-2018 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
AD FUTURA. Science and Education Foundation of the Republic of Slovenia, | AD FUTURA |
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https://www.mdpi.com/1420-3049/28/23/7830 |
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=152740 |
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Milosheska, Daniela, farmacevtka | 38803 |
Roškar, Robert, 1975- | 23549 |
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