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Characterisation and expression of a gene encoding a mutarotase from the fungus Rhizopus nigricansVilfan, Tanja ...A gene coding for a mutarotase was isolated and characterised from the filamentous fungus Rhizopus nigricans. In order to determine the encoded enzyme's activity a recombinant protein was prepared in ... the baculovirus expression system and the mutarotase activity was determined. Expression studies showed that the gene is repressed by high as well as low concentrations of glucose and derepressed during deficiency of glucose. Besides the regulation at the level of transcription, an accelerative effect of glucose in growth medium on the mutarotase mRNA decay was also demonstrated. Moreover, a Southern hybridisation performed at lower temperatures suggested that the R. nigricans genome harbours a nucleotide sequence, that is homologous to the isolated gene.Source: FEMS microbiology letters. - ISSN 0378-1097 (Letn. 235, 2004, str. 101-108)Type of material - article, component partPublish date - 2004Language - englishCOBISS.SI-ID - 17668825
Author
Vilfan, Tanja |
Črešnar, Bronislava, 1950-2024 |
Fournier, Didier |
Stojan, Jure, 1956- |
Breskvar, Katja
Topics
Rhizopus |
Enzymology |
Genetics |
Galactose |
Metabolism |
Uridine Diphosphate Glucose Epimerase |
Gene Expression Regulation, Fungal |
Recombinant Fusion Proteins |
Glucose |
Transcription, Genetic |
Genome, Fungal |
Genomic Library |
Hybridization |
Base Sequence |
Polymerase Chain Reaction |
Dna, Complementary |
Cloning, Molecular |
Galaktoza |
Gensko izražanje, regulacija glivična |
Rekombinantne, fuzijske beljakovine |
Rhizopus |
Baze, zaporedje |
DNA komplementarna |
Genom gliv |
Genomska knjižnica |
Glukoza |
Hibridizacija |
Kloniranje molekulsko |
Polimerazna, verižna reakcija |
Transkripcija genetična
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Vilfan, Tanja | 19132 |
Črešnar, Bronislava, 1950-2024 | 08777 |
Fournier, Didier | ![]() |
Stojan, Jure, 1956- | 03723 |
Breskvar, Katja | 03079 |
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