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MIBlood-EV [Elektronski vir] : minimal information to enhance the quality and reproducibility of blood extracellular vesicle researchLucien, Fabrice ...Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but ... also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood-EV), a tool to record and report information about pre-analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre-analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence-based optimization of pre-analytical protocols through meta-analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter-laboratory comparative EV studies, and (iv) improve the peer review process.Source: Journal of extracellular vesicles [Elektronski vir]. - ISSN 2001-3078 (Vol. 12, iss. 12, [article no.] e12385, 2023, str. 1-10)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 179034883
Author
Lucien, Fabrice |
Gustafson, Dakota |
Lenassi, Metka, 1978- |
Li, Bo, biokemik |
Teske, Jacob J. |
Boilard, Eric |
Clemm von Hohenberg, Katharina |
Falcon-Perez, Juan Manuel |
Gualerzi, Alice |
Reale, Antonia
Topics
biomarker |
blood |
extracellular vesicles |
liquid biopsy |
quality control |
reproducibility |
standardization |
biološki označevalec |
kri |
zunajcelični vezikli |
biopsija tekočine |
kontrola kakovosti |
ponovljivost |
standardizacija
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Lucien, Fabrice | ![]() |
Gustafson, Dakota | ![]() |
Lenassi, Metka, 1978- | 24288 |
Li, Bo, biokemik | ![]() |
Teske, Jacob J. | ![]() |
Boilard, Eric | ![]() |
Clemm von Hohenberg, Katharina | ![]() |
Falcon-Perez, Juan Manuel | ![]() |
Gualerzi, Alice | ![]() |
Reale, Antonia | ![]() |
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