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An LC-MS/MS method for quantification of lamotrigine and its main metabolite in dried blood spots [Elektronski vir]Milosheska, Daniela, farmacevtka ...Background: The antiepileptic drug lamotrigine (LTG) shows high pharmacokinetic variability due to genotype influence and concomitant use of glucuronidation inducers and inhibitors, both of which may ... be frequently taken by elderly patients. Our goal was to develop a reliable quantification method for lamotrigine and its main glucuronide metabolite lamotrigine-N2-glucuronide (LTG-N2-GLU) in dried blood spots (DBS) to enable routine therapeutic drug monitoring and to identify altered metabolic activity for early detection of drug interactions possibly leading to suboptimal drug response. Results: The analytical method was validated in terms of selectivity, accuracy, precision, matrix effects, haematocrit, blood spot volume influence, and stability. It was applied to a clinical study, and the DBS results were compared to the concentrations determined in plasma samples. A good correlation was established for both analytes in DBS and plasma samples, taking into account the haematocrit and blood cell-to-plasma partition coefficients. It was demonstrated that the method is suitable for the determination of the metabolite-to-parent ratio to reveal the metabolic status of individual patients. Conclusions: The clinical validation performed confirmed that the DBS technique is a reliable alternative for plasma lamotrigine and its glucuronide determination.Source: Pharmaceuticals [Elektronski vir]. - ISSN 1424-8247 (Vol. 17, no. 4, [Article no.] 449, 2024, 16 str.)Type of material - e-article ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 191424771
Author
Milosheska, Daniela, farmacevtka |
Roškar, Robert, 1975- |
Vovk, Tomaž, 1972- |
Lorber, Bogdan |
Grabnar, Iztok |
Trontelj, Jurij
Topics
Epileptični napadi |
Farmakokinetika |
posušena krvna lisa |
lamotrigin |
lamotrigin glukuronid |
terapevtsko spremljanje zdravil |
klinična validacija |
učinek hematokrita |
dried blood spot |
lamotrigine |
lamotrigine glucuronide |
therapeutic drug monitoring |
clinical validation |
haematocrit effect
Author | Milosheska, Daniela, farmacevtka ... |
Title | An LC-MS/MS method for quantification of lamotrigine and its main metabolite in dried blood spots [Elektronski vir] |
Publication date | 2024-03-30 |
COBISS.SI-ID | 191424771 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih | P1-0189-2018 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Link |
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https://www.mdpi.com/1424-8247/17/4/449 |
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=155604 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Milosheska, Daniela, farmacevtka | 38803 |
Roškar, Robert, 1975- | 23549 |
Vovk, Tomaž, 1972- | 18155 |
Lorber, Bogdan | 12152 |
Grabnar, Iztok | 16107 |
Trontelj, Jurij | 23420 |
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