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Safety and efficacy of IL-12 plasmid DNA transfection into pig skin [Elektronski vir] : supportive data for human clinical trials on gene therapy and vaccinationLampreht Tratar, Urša ...Gene electrotransfer (GET) of plasmids encoding interleukin 12 (IL-12) has already been used for the treatment of various types of tumors in human oncology and as an adjuvant in DNA vaccines. In ... recent years, we have developed a plasmid encoding human IL-12 (phIL12) that is currently in a phase I clinical study. The aim was to confirm the results of a non-clinical study in mice on pharmacokinetic characteristics and safety in a porcine model that better resembled human skin. The GET of phIL12 in the skin was performed on nine pigs using different concentrations of plasmid phIL12 and invasive (needle) or noninvasive (plate) types of electrodes. The results of our study demonstrate that the GET of phIL-12 with needle electrodes induced the highest expression of IL-12 at the protein level on day 7 after the procedure. The plasmid was distributed to all tested organs; however, its amount decreased over time and was at a minimum 28 days after GET. Based on plasmid copy number and expression results, together with blood analysis, we showed that IL-12 GET is safe in a porcine animal model. Furthermore, we demonstrated that pigs are a valuable model for human gene therapy safety studies.Source: International journal of molecular sciences [Elektronski vir]. - ISSN 1422-0067 (Vol. 25, no. 6, 2024, str. 3151-1-3151-12)Type of material - e-article ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 191795459
Author
Lampreht Tratar, Urša |
Jesenko, Tanja |
Omerzel, Maša, mag. farm. |
Seliškar, Alenka |
Stupan, Urban, štud. med. |
Djokić, Mihajlo |
Sredenšek, Jerneja |
Trotovšek, Blaž |
Serša, Gregor |
Čemažar, Maja
Topics
interlevkin 12 |
elektroprenos genov |
imunoterapija |
interleukin 12 |
gene electrotransfer |
immunotherapy
Author | Lampreht Tratar, Urša ... |
Title | Safety and efficacy of IL-12 plasmid DNA transfection into pig skin [Elektronski vir] : supportive data for human clinical trials on gene therapy and vaccination |
Publication date | 2024-03-09 |
COBISS.SI-ID | 191795459 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Unidentified |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev | P3-0003-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih | P4-0053-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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APC – Article Processing Charge | 3324.35 € |
3324.35 € : Onkološki inštitut Ljubljana |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Lampreht Tratar, Urša | 36367 |
Jesenko, Tanja | 33227 |
Omerzel, Maša, mag. farm. | 34373 |
Seliškar, Alenka | 18593 |
Stupan, Urban, štud. med. | |
Djokić, Mihajlo | 30463 |
Sredenšek, Jerneja | 56386 |
Trotovšek, Blaž | 15895 |
Serša, Gregor | 08800 |
Čemažar, Maja | 14575 |
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