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Učinkovanje kromovih (VI) spojin na izbrane kvasovke rodov: Saccharomyces, Schwanniomyces, Candida : diplomska naloga = Effect of chromium (VI) compounds on selected yeasts from genera: Saccharomyces, Schwanniomyces, Candida : graduation thesis
Jamnik, Polona, biotehnologinja
V nalogi smo proučevali učinkovanje Cr(VI) spojin: K2Cr2O7, K2CrO4, KCrClO3, Na2Cr2O7 x 2 H2O, (NH4)2Cr2O7 na kvasovke: Saccharomyces cerevisiae - ZIM 753, Saccharomyces cerevisiae - ZIM 156, ...Saccharomyces cerevisiae - M1, Saccharomyces paradoxus - ZIM 754, Saccharomyces bazanus - ZIM 749, Saccharomyces pastorianus - ZIM 752, Saccharomyces diastaticus - ZIM 152, Candida utilis - ZIM 70, Schwanniomyces occidentalis - ZIM 763. Koncentracije Cr(VI) so bile 0, 1, 10, 100 in 1000 [mikro]mol/l. Učinkovanje Cr(VI) spojin na kvasovke je potekalo 24 ur v pufru MES (2-morfolinoetansulfonska kislina) pri temperaturi 28 °C. Po 24 urah smo kvasno suspenzijo v pufru z različnimi koncentracijami Cr(VI) ustrezno razredčili in prenesli na SA gojišče, kjer smo po inkubaciji pri temperaturi 28 °C s štetjem kolonij določili živost kvasovk. Rezultati so pokazali, da so razlike v toksičnosti raličnih Cr(VI) spojin statistično značilne samo pri določenih koncentracijah Cr(VI) in določenih spojinah, odvisno od posameznih kvasovk. Ugotovili smo tudi, da obstajajo razlike v toleranci posameznih kvasovk na Cr(VI) spojine. Najbolj tolerantne so bile: Saccharomyces cerevisiae - ZIM 753, Saccharomyces cerevisiae - ZIM 156, Saccharomyces cerevisiae - M1, Saccharomyces paradoxus - ZIM 754.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [P. Jamnik], 1997
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