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Focused ion beam (FIB) / SEM system for in situ manipulation and microscopy of crustacean digestive system (Isopoda, Crustacea)
Drobne, Damjana ...
The focused ion beam (FIB) / SEM system was used to prepare cross-sections of selected regions of digestive gland epithelium of Porcellio scaber (Isopoda, Crustacea) for scanning electron microscopy. ...Application of a FIB / SEM systemfor morphological studies has advantages over conventional microscopy techniques because of: in situ site-specific 2D-sectioning and imaging of subsurface microstructures; no need to embed the sample prior to sectioning; and a possibility of using a wide range of magnifications while imaging the same sample. The FIB / SEM system is in the early stage of application in lifesciences and appears to be a technique of choice for 3D-observations at the organism, organ, tissue and (sub)cellular levels.
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