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Customized 16S-23S rDNA ITS amplicon metagenomics for acetic acid bacteria species identification in vinegars and kombuchas [Elektronski vir]Ribič, Alja ; Trček, JanjaAcetic acid bacteria (AAB) are involved in food and beverage production bioprocesses, like those in vinegar and kombucha. They oxidize sugars and alcohols into various metabolites, resulting in the ... final products’ pleasant taste and aroma. The 16S rDNA amplicon metagenomics using Illumina technology is usually used to follow the microbiological development of these processes. However, the 16S rRNA gene sequences among different species of AAB are very similar, thus not enabling a reliable identification down to the species level but only to the genus. In this study, we have constructed primers for amplifying half of the 16S-23S rRNA gene internal transcribed spacer (ITS) for library construction and further sequencing using Illumina technology. This approach was successfully used to estimate the relative abundance of AAB species in defined consortia. Further application of this method for the analysis of different vinegar and kombucha samples proves it suitable for assessing the relative abundance of AAB species when these bacteria represent a predominant part of a microbial community.Source: Microorganisms [Elektronski vir]. - ISSN 2076-2607 (Vol. 12, iss. 5, [article no.] 1023, 2024, 8 str.)Type of material - e-article ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 196373763
Author
Ribič, Alja |
Trček, Janja
Topics
Mikrobiologija |
Bakterije |
mikrobiota |
konzorciji |
ITS metagenomics |
microbiota |
consortia |
Acetobacter |
Komagataeibacter |
Novacetimonas
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Author | Ribič, Alja ; Trček, Janja |
Title | Customized 16S-23S rDNA ITS amplicon metagenomics for acetic acid bacteria species identification in vinegars and kombuchas [Elektronski vir] |
Publication date | 2024-05-18 |
COBISS.SI-ID | 196373763 |
Publication version in repository | Unidentified |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Unidentified |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
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Fizikalno kemijski pojavi na površinskih plasteh in uporaba nanodelcev | P2-0006-2018 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Razvoj raziskovalne infrastrukture za mednarodno konkurenčnost slovenskega RRI prostora - RI-SI-LifeWatch | C3330-19-952062 |
European Regional Development Fund Ministrstvo za izobraževanje, znanost in šport |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Ribič, Alja | ![]() |
Trček, Janja | 14279 |
Source: Personal bibliographies
and: SICRIS
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