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Proučevanje razvoja in sestave črevesne mikrobne populacije dojenčka na osnovi analiz blata : diplomska naloga = The study of the establishment and development of the intestinal microflora of an breast fed infant : graduation thesis
Zabukošek, Martina
Proučevali smo sestavo in razvoj črevesne mlečnokislinske mikrobne populacije novorojenčka, hranjnega izključno z dojenjem. Vzorce blata smo jemali med prvim in triindvajsetim tednom starosti. Za ...izolacijo bakterij iz blata dojenčka smo uporabili različna selektivna gojišča (MRS, MRS z dodatkom NNLP, MRS pH 5.4, Rogosa, Rogosa z dodatkom X-glu, M 17, Chalmer) in ugotavljali njihovo selektivnost. V gramu blata teden dni starega dojenřka je bilo 4.0 x 10 exp8 bakterij. Število je s časom naraščalo in deveti teden doseglo najvišjo vrednost (4.2 x 10 exp10 bakterij/g blata). Z metodo neposredne inhibicije s spoti na agarju smo proučevali protibakterijski učinek 130 izolatov, od skupno 140, proti sedmim indikatorskim bakterijam. S pomočjo proteolitičnega encima tripsina z metodo luknjic na trdnem gojišču smo ugotovili, da protibakterijsko delovanje izolatov ni posledica delovanja metabolitov proteinske narave. 25 izolatov smo identificirali z osnovnimi testi, 10 od njih z API 50 CHL testi in 10 od njih s fruktoza-6-fosfat fosfoketolaznim testom. V blatu dojenčka so prevladovale vrste Lactobacillus plantarum (L. plantarum), Lactobacillus acidophilus (L. acidophilus), Lactococcus lactis supsp. lactis (L. lactis supsp. lactis), Lactococcus raffinolactis (L. raffinolactis), Lactobacillus paracasei subsp. paracasei (L.paracasei subsp. paracasei) in Enterococcus faecalis (E. faecalis). Prisotnost bifidobakterij nismo ugotovili.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [M. Zabukošek], 1997
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