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Nanobiocatalyst based on covalent immobilization of proteases onto functionalized nanocellulose for efficient production of sunflower meal protein hydrolysates [Elektronski vir]Katić, Katarina ...This study aimed to engineer bio-based and environmentally friendly nanobiocatalyst systems using commercially available protease preparations, Alcalase® 2.4L (Alcalase) and Flavourzyme® ... (Flavourzyme), along with functionalized cellulose nanocrystals (CNC). The study highlights the advantageous properties of the obtained glutaraldehyde-activated amino-modified CNC support (GA-amino-CNC), characterized as nanorods with dimensions ranging between 100 and 800 nm in length and 40–60 nm in width, particularly for protease immobilization. Maximum specific activity of immobilized Alcalase (15 IU/mg of proteins) was achieved at an initial enzyme concentration of 52 mg/g of support after 3 h, with 80% of covalently attached enzyme molecules. In the case of Flavourzyme, maximum exo-hydrolytic activity of 21 L-pNAU/g of support was observed after 20 min, while a higher yield of covalently bound enzyme molecules (53%) detected after 1 h. The covalently bound proteases on glutaraldehyde-activated amino-modified cellulose nanocrystals were evaluated for their efficacy in producing high-quality sunflower-based protein hydrolysates suitable for food and feed applications. Sequential hydrolysis of sunflower meal protein isolate (SMPI) using the developed nanobiocatalysts yielded a maximum hydrolysis degree of 20%. In addition, more than 80% of the initial activity of nanobiocatalysts were retained after 3 reuses, suggesting that these protease nanobiocatalysts have potential application in production of SMP hydrolysates.Source: Food bioscience [Elektronski vir]. - ISSN 2212-4306 (Vol. 61, [article no.] 104590, Oct. 2024, str. 1-10)Type of material - e-article ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 199780611
Author
Katić, Katarina |
Banjanac, Katarina |
Simović, Milica |
Veljković, Milica, kemičarka |
Ćorović, Marija |
Denić, Lara |
Zakić, Teodora |
Kralj, Slavko, 1983- |
Bezbradica, Dejan
Topics
Funkcionalna živila |
Celuloza |
nanoceluloza |
nanocellulose |
nanobiocatalysts |
cellulose nanocrystals |
protein hydrolysates |
by-products
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Katić, Katarina | ![]() |
Banjanac, Katarina | ![]() |
Simović, Milica | ![]() |
Veljković, Milica, kemičarka | ![]() |
Ćorović, Marija | ![]() |
Denić, Lara | ![]() |
Zakić, Teodora | ![]() |
Kralj, Slavko, 1983- | 29529 |
Bezbradica, Dejan | ![]() |
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