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Določanje ToMV virusa v vodah s PCR v realnem času = Detection of ToMV virus in water using real-time PCR
Boben, Jana ...
Irrigation waters can represent a source of infection for plants as plant viruses can enter the plants through the root system and cause disease symptoms on host plants. Viruses can also be released ...from infected plants into a drainage water and can spread to the neighbouring plants. Concentrationof plant viruses in irrigation waters is usually below the sensitivity of frequently used detection methods such as ELISA. ToMV (Tomato mosaic virus) was used as a model for development of a highly specific and more sensitive real-time PCR assay. ToMV is known to cause symptoms on Nicotiana glutinosa in concentrations below the sensitivity of ELISA. Newly developed method is around 1000-times more sensitive and enables us to detect the viruses in much lower concentrations. CIMr Convective Interaction Media disk monolithic columns were also used in order to concentrate water samples where the virus concentration was below the detection limit of real-time PCR. They proved to be successful in concentrating the water samples where virus concentration was below the detection limit of real-time PCR. Combination of concentrating procedure using CIM monolithic chromatographic supports and detection of viruses using a more sensitive method can be used to effectively monitor the condition of irrigation waters and to use the information about the health status of waters in intensive plant production.
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