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Virusi na paradižniku v Sloveniji = Viruses infecting tomato in Slovenia
Mehle, Nataša, biologinja ; Tušek-Žnidarič, Magda ; Ravnikar, Maja
Diagnostic laboratory at the National Institute of Biology is performing laboratory testing of viruses on vegetables and ornamentals, in the frame of amonitoring of quarantine and harmful pathogens ...carried out by the Phytosanitary Adminstration and Phytosanitary Inspection Service of the Republic of Slovenia. During last few years most frequently found viruses on tomato were CMV (Cucumber mosaic virus) and PVY (Potato virus Y). Occasionally, AMV (Alfalfa mosaic virus), TMV (Tobacco mosaic virus), ToMV (Tomato mosaic virus) and TSWV (Tomato spotted wilt virus), were found. Other,not identified particles were observed by electron microscopy, with the morphology corresponding to Cucumoviruses, Geminiviruses and Tobamoviruses. Quarantine pests, such as PepMV (Pepino mosaic virus), TYLCV (Tomato yellow leaf curl virus) and ToRSV (Tomato ringspot virus) have not been found, yet. CMV and PVY were often found in mixed infection, as well as in combinations with other viruses, for example a combination of PVY and TSWV was found, causing necrosis on stem and petioles, and leaves curling. ToMV, found in one tomato glasshouse in 2004, causing wilting and necrosis, the leaves were also wrinkled and chlorotic. In 2003, we analysed tomato plant expressing unusual symptoms of distinct chlorotic spots and wrinkles on fruits, and narrow, necrotic leaves. No above mentioned viruses were found by ELISA. Electron microscopy revealed very clear Rhabdoviridae particles and isometric particlesof an unknown identity in sap of mechanically inoculated test plants Nicotiana tabacum cv. White Burley, Nicotiana clevelandii and Nicotiana benthamiana.
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