Imobilizacija encima celulaza v zamrežene encimske skupke (CLEAs) ter vpliv superkritičnega ogljikovega dioksida na njihovo aktivnost : diplomsko delo

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Imobilizacija encima celulaza v zamrežene encimske skupke (CLEAs) ter vpliv superkritičnega ogljikovega dioksida na njihovo aktivnost : diplomsko delo

Pogorelc, Katja, 1985-

Diplomsko delo opisuje sintezo encimskih skupkov iz encima celulaza ali na kratko CLEAs ter vpliv superkritičnega ogljikovega dioksida (SC CO2) na aktivnost teh skupkov. Priprava zamreženih encimskih ... skupkov poteka po določenem postopku, katerega ključna dela sta obarjanje in zamreženje. Torej obarjanje topnega encima z ustreznim organskim topilom in nadaljnjo zamreženje tako oborjenega encima s pomočjo mrežnega povezovalca. V našem primeru smo kot mrežni povezovalec uporabili glutaraldehid in aceton kot obarjalni reagent. Zamrežene encimske skupke (CLEAs) smo izpostavili pri temperaturah 35 °C, 40 °C, 50 °C in 60 °C. Istočasno smo jih izpostavili v SC CO2 v visokotlačnem šaržnem reaktorju, in sicer pri temperaturah 35 °C, 40 °C, 50 °C in 60 °C in tlaku 100 bar ter pri temperaturah 35 °C in 40 °C ter tlaku 200 bar. Aktivnost CLEAs je s podaljšanjem inkubacijskega časa upadala.

Type of material - undergraduate thesis ; adult, serious

Publication and manufacture - Maribor : [K. Pogorelc], 2014

Language - slovenian

COBISS.SI-ID - 20611848

Link(s):
Digital Library of the University of Maribor – DLUM

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Author
Pogorelc, Katja, 1985-

Availability in libraries

Library/institution	City	Acronym	For loan	Other holdings
Miklošič Library FPNM, Maribor	Maribor	PEFMB	reading room 1 cop.

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Year	Impact factor		Edition		Category		Classification
Year	JCR	SNIP	JCR	SNIP	JCR	SNIP	JCR	SNIP

Links to authors' personal bibliographies	Links to information on researchers in the SICRIS system
Pogorelc, Katja, 1985-
Leitgeb, Maja	00534

Source: Personal bibliographies and: SICRIS

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