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Imunski odziv miši na imunizacijo z Lactobacillus acidophilus LF221 in Lactobacillus casei ATCC 393 : magistrsko delo = Immune response of mice immunized with Lactobacillus acidophilus LF221 and Lactobacillus casei ATCC 393 : master of science thesis
Hočevar, Irena, živilska tehnologinja
Raziskava proučuje vpliv Lactobacillus acidophilus LF221 in Lactobacillus casei ATCC 393 na protitelesni imunski odziv miši NIH. Miši smo imunizirali intraperitonalno (IP.) in oralno z odmerkom 2,4 x ...1010 živih ali mrtvih bakterijskih celic. 7., 14., 18., 21. in 24. dan po prvi imunizacij smo odvzeli vzorce krvi in z DIBA in ELISA metodama spremljali nastanek celotnih in anti-laktobacili IgA, IgG in IgM protiteles. Iz rezultatov smo ugotovili, da je koncentracija anti-laktobacili protiteles zelo majhna v primerjavi s celotnimi protitelesi. Pri obeh načinih imunizacije nismo zaznali značilnih razlik v koncentraciji IgA protiteles med kontrolno in testiranimi skupinami. Po IP. imunizaciji smo ugotovili največjo koncentracijo IgG in IgM protiteles in sicer pri imunizaciji z živimi celicami seva LF221 (bac+). Iz tega smo sklepali, da verjetno bakteriocinski kompleks, vezan na celično steno, deluje kot antigen. Ugotovili smo tudi, da sta krivulji sinteze specifičnih serumskih IgG in IgM protiteles po IP. imunizaciji značilni za nastanek imunskega spomina. Krivulji sta bolj izraziti pri imunizaciji z živimi celicami. Imunski odziv pri oralni imunizaciji je v primerjavi z IP. imunizacijo precej manjši, saj je predvsem posledica aktivacije sekretornega imunskega sistema.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [I. Plantarič], 1998
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