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Priprava s peroksidazo konjugiranih monoklonskih protiteles proti kokošjim imunoglobulinom razreda G (IgY) : diplomska naloga = Preparation of peroxidase conjugated monoclonal antibodies against G class of chicken immunoglobulins (IgY) : graduation thesis
Rejc, Mateja
OkuÍbe perutnine v intenyivni reji so relativno pogoste in lahko povyrořajo veliko ekonomsko Ǵkodo. Veřino okuÍb lahko ugotovimo s seroloǴkimi testi, ki temeljijo na dokayovanju specifiřnih serumskih ...imunoglobulinov rayreda Z (IgZ), ki so ptiřji homolog sesalskemu rayredu G (IgG). Poleg ustreynega antigena so ya iyvedbo takǴnega testa potrebna protitelesa proti molekuli IgZ,ki so lahko poliklonskega ali monoklonskega iyvora. Iy jajřnega rumenjaka kokoǴi smo iyolirali IgZ, y njimi imuniyirali miǴ BALB/c in pripravili monoklonska protitelesa (mAb) proti kokoǴjim IgZ - mAb M2 -, ki so reagirala tudi y determinantami na IgZ fayana, purana in pava. Determinanta, ki jo prepoynavajo mAb M2, se nahaja v konstantni regiji teÍke verige kokoǴjih IgZ in ni glikoyilirana. Pridobljenim mAb M2 smo dolořili pH optimum, optimalno delovno koncentracijo v direktnem imunoencimskem testu, in ocenili afiniteto veyave. MAb M2 smo konjugirali s hrenovo peroksidayo in konjugat M2-p testirali v veř rayliřnih testih. M2-p je potencialno uporaben ya seroloǴko diagnostiko okuÍb kokoǴi, puranov, fayanov in pavov. Ker takǴnega konjugata natrÍiǴřu nismo yasledili, smo ya morebitno ponudbo iydelali specifikacijski list.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [M.Rejc] ; BF, Enota medoddelčnega študija mikrobiologije, 1999
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