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  • Vitalnost spermatozoida čovjeka nakon vitrifikacije u tekućem dušiku = Viability of human sprematozoa after vitrification in liquid nitrogen
    Vlaisavljević, Veljko ; Kovačič, Borut
    The authors report on a simple technique of storing the seminal fluid in fiqusd nitrogen. A glycerol based medium without yolk is used as cryoprotectant. The specimens (n=75) were stored in a ... tuberculin syringe. After thawing, the semen motility dropped from 60.6 û 16.1 per cent to 27.5 û 16.3 per cent. The sperm motility after thawing was better (32.6 û 14.7 per cent) in the eʼaculate with more than 50 mill/ml motile sperms than in the ejaculate with less than 50 mill/ml (18.4 per cent û 14.7 per cent). Using the swim-up technique, the separation of motile sperms from the thawed specimen was successful in cases where the native ejaculate contained more than 50 mil/ml of spermatozoa.
    Source: Gynaecologia et Perinatologia : journal for gynaecology, perinatology, reproductive medicine and ultrasonic diagnostics. - ISSN 1330-0091 (Letn. 2, št. 1, 1993, str. 1-3)
    Type of material - article, component part
    Publish date - 1993
    Language - croatian
    COBISS.SI-ID - 2319833

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