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Določanje populacije kvasovk v različnih stopnjah proizvodnje kefirja : diplomska naloga = Identification of yeast population in different stages of kefir production : graduation thesisJuteršek, BorutNamen poskusa je bil ugotoviti vrstno sestavo populacije kvasovk, prisotnih v kefirnih yrnih, kefirju druge in tridesete ǴarÍe. Iy vyorcev je bilo iyoliranih 91 iyolatov kvasovk in analiyiranih y ... metodo PCR - ribotipiyacije. Na osnovi dobljenih restrikcijskih profilov so bili analiyirani iyolati raydeljeni v dve skupini. Raydelitev je bila potrjena y opayovanjem morfologije vegetativnih celic. Skupini sta bili identificirani s primerjavo restrikcijskih profilov referenřnih tipskih sevov, pravilnost identifikacije pa potrjena y biokemijskimi in fiyioloǴkimi testi. Prisotnost spor je bila preverjena y rastjo iyolatov kvasovk na dolořenih sporulacijskih gojiǴřih in mikroskopskim opayovanjem preparatov. S testom yimocidne aktivnosti je bila testirana sposobnost iyolatov iylořati yimocine. Raylikovanje kvasovk ynotraj vrst je bilo narejeno y elektroforetsko kariotipiyacijo. Vsi iyolati iy kefirnih yrn so bili identificirani kot neynana kvasovka U. Pri kefirju druge ǴarÍe je bilo 89% iyolatov identificiranih kot neynana kvasovka U in 11% kot vrsta Kluzveromzces marxianus. Pri kefirju tridesete ǴarÍe so bili vsi iyolatiidentificirani kot vrsta K. marxianusType of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [B.Juteršek] ; BF, Enota medoddelčnega študija mikrobiologije, 1999Language - slovenianCOBISS.SI-ID - 2326392
Author
Juteršek, Borut
Other authors
Raspor, Peter
Topics
kvasovke |
kefir |
kefirna zrna |
populacija kvasovk |
identifikacija kvasovk |
PCR ribotipizacija |
kariotipizacija |
fiziološki testi |
biokemijski testi |
morfologija spor |
kvasovka U |
Kluyveromyces marxianus |
morfologija vegetativnih celic |
morfologija vegetativnih celic |
yeasts |
kefir |
kefir grains |
yeast population |
yeast identification |
PCR ribotyping |
karyotyping |
physiological tests |
biochemical tests |
yeast U |
Kluyveromyces marxianus |
morphology of spores |
morphology of vegetative cells |
morphology of vegetative cells
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BF, Department of Food Science and Technology, Lj. | Ljubljana | BFZIV |
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Juteršek, Borut | ![]() |
Raspor, Peter | 04001 |
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