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Določanje populacije kvasovk v različnih stopnjah proizvodnje kefirja : diplomska naloga = Identification of yeast population in different stages of kefir production : graduation thesis
Juteršek, Borut
Namen poskusa je bil ugotoviti vrstno sestavo populacije kvasovk, prisotnih v kefirnih yrnih, kefirju druge in tridesete ǴarÍe. Iy vyorcev je bilo iyoliranih 91 iyolatov kvasovk in analiyiranih y ...metodo PCR - ribotipiyacije. Na osnovi dobljenih restrikcijskih profilov so bili analiyirani iyolati raydeljeni v dve skupini. Raydelitev je bila potrjena y opayovanjem morfologije vegetativnih celic. Skupini sta bili identificirani s primerjavo restrikcijskih profilov referenřnih tipskih sevov, pravilnost identifikacije pa potrjena y biokemijskimi in fiyioloǴkimi testi. Prisotnost spor je bila preverjena y rastjo iyolatov kvasovk na dolořenih sporulacijskih gojiǴřih in mikroskopskim opayovanjem preparatov. S testom yimocidne aktivnosti je bila testirana sposobnost iyolatov iylořati yimocine. Raylikovanje kvasovk ynotraj vrst je bilo narejeno y elektroforetsko kariotipiyacijo. Vsi iyolati iy kefirnih yrn so bili identificirani kot neynana kvasovka U. Pri kefirju druge ǴarÍe je bilo 89% iyolatov identificiranih kot neynana kvasovka U in 11% kot vrsta Kluzveromzces marxianus. Pri kefirju tridesete ǴarÍe so bili vsi iyolatiidentificirani kot vrsta K. marxianus
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [B.Juteršek] ; BF, Enota medoddelčnega študija mikrobiologije, 1999
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