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Molekularno biološko odkrivanje nekaterih bakterijskih skupin v prebavilih laboratorijskih podgan : diplomska naloga = Molecular biological detection of some bacterial species in intestine of laboratory rats : graduation thesis
Pokorny, Vesna
V prehranskem poskusu smo samce poskusnih podgan krmili s polsintetiřno krmo ydodatkom surovega fiÍola, kontrolno skupino podgan pa y obiřajno krmno meǴanico. Ob koncu poskusa se je kontrolni skupini ...teÍa v povpreřju poveřala ya 5.84 g, poskusni pa v povpreřju ymanjǴala ya 9.1 g. Y veriÍno reakcijo s polimerayo DNK smo s specifiřnimi yařetnimi oligonukleotidi semi-kvantitativno pomnoÍevali gene ya 16S rRNK iy rayliřnih redřitev DNK, iyoliranih iy tankega řrevesa podgan. Potrdili smo preliminarne ugotovitve o poveřanju Ǵtevila bifidobakterij in predstavnikov ?-podskupine proteobakterij v tankem řrevesu poskusnih podgan. Potrdili smo tudi prisotnost predstavnikov filogenetske skupine Cztophaga-Flexibacter-Bacteroides v anteriornem in posteriornem delu tankega řrevesa kontrolnih in poskusnih podgan. Pripravili smo tudi strategijo ya iyvedbo kompetitivne in specifiřne veriÍne reakcije s polimerayo, ki bi omogořala hitro in natanřno Ǵtetje bifidobakterij v vyorcih podganjih řreves.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [V.Pokorny] ; [BF, Enota medoddelčnega študija mikrobiologije], 1999
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