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Phylogenetic analysis confirms hepatitis C virus transmission among hemodialysis patients in KosovoJakupi, Xhevat ...Introduction: It has recently been demonstrated that there i s a very high prevalence of hepatitis C virus (HCV) infection among hemodialysis patients in Kosovo with HCV subtype 1 being the most ... prevalent subtype. In this study, we further detail the molecular epidem iology of HCV outbreaks occurring in seven dialysi s centers in Kosovo. Methodology: In total, 273 samples obtained from HCV RNA positive patients undergoing hemodialysis at one of the seven center s in Kosovo were selected for this study: 171 subtype 1a samples, 91 subtype 4d samples, and 11 subtype 1b sa mples. A partial HCV NS5B region was amplified and sequenced. Subtype - specific phylogenetic analyses were performed with the inclusion of control sequences and transmission clusters were identified. Results: NS5B sequences were successfully obtained in 257 /273 (94.1%) of samples; 162 subtype 1a, 84 subtype 4d, and 11 subtype 1b sequences. Phylogenetic analyses showed a high degree of phylogenetic clustering of HCV sequences subtyped 1a (99.4%), 1b (63 .6%), and 4d (76.2%). Distinct phylogenetic clusters of s equences obtained from hemodialysis patients were observed for all three subtypes studied. In addition, several smaller clusters within the large clusters were identified, mainly from a single dialysis center. Conclusions: Phylogenetic analyses confirmed nosocomial transmission during dialysis as a major factor in the spread of HCV at the seven dialysis centers in Kosovo.Source: Journal of infection in developing countries. - ISSN 2036-6590 (Vol. 13, no. 12, 2019, str. 1142 - 1149)Type of material - article, component part ; adult, seriousPublish date - 2019Language - englishCOBISS.SI-ID - 23680259
Author
Jakupi, Xhevat |
Mlakar, Jana, biol., 1982 |
Lunar, Maja M., 1982- |
Rudhani, Ibrahim |
Raka, Lul |
Tavakoli, Norma P. |
Poljak, Mario, 1965-
Topics
HCV |
phylogenetic analysis |
dialysis |
HCV |
filogenetska analiza |
dializa
source: Journal of infection in developing countries. - ISSN 2036-6590 (Vol. 13, no. 12, 2019, str. 1142 - 1149)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Jakupi, Xhevat | |
Mlakar, Jana, biol., 1982 | 57043 |
Lunar, Maja M., 1982- | 54554 |
Rudhani, Ibrahim | |
Raka, Lul | |
Tavakoli, Norma P. | |
Poljak, Mario, 1965- | 11622 |
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