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Adhesion molecules involved in the binding and subsequent engulfment of thymocytes by a rat thymic epithelial cell lineČolić, Miodrag ...A rat thymic epithelial cell (TEC) line (R-TNC.1) was established from a long-term TEC culture. Based on its ultrastructure, phenotype and cytokeratin profile, this line was characterized as a type ... of cortical TEC. R-TNC.1 cells had nursing activity which was manifested by the binding and subsequent engulfment of thymocytes. The role of adhesion molecules involved in these processes was studied extensively using a coculture of resting thymocytes and unstimulated or interferon-gamma (IFN-gamma)-stimulated R-TNC.1 cells. It was found that a number of adhesion molecules, such as CD2, CD4, CD8, LFA-1, CD18,ICAM-1 and Thy-1, was partly involved in the nursing activity. The effect of monoclonal antibodies (mAb) to these molecules depended on the incubation time and stimulation of R-TNC.1 cells. The inhibitory effect of mAbto CD2, LFA-1, CD18 and ICAM-1 on thymocyte engulfment was higher than their effect on thymocyte binding to the R-TNC.1 line. In addition, a LFA-1/CD18-dependent/ICAM-1-independent adhesion pathway was identified when unstimulated R-TNC.1 cells with minimal expression of ICAM-1 were used. The combination of inhibitory mAb did not completely abrogate the nursing activityof the R-TNC.1 line, suggesting the possible involvement of some other adhesion molecules.Source: Immunology. - ISSN 0019-2805 (Letn. 83, št. 3, 1994, str. 449-456)Type of material - article, component partPublish date - 1994Language - englishCOBISS.SI-ID - 23943129
Author
Čolić, Miodrag |
Vučević, Dragana |
Miyaska, Masayuki |
Tamatani, Takuya |
Pavlović, Miloš, 1966- |
Dujić, Aleksandar
Topics
Animals |
Antibodies, Monoclonal |
Cell Adhesion Molecules |
Physiology |
Cell Line |
Epithelium |
Metabolism |
Ultrastructure |
Fluorescent Antibody Technique |
Microscopy, Electron |
Phenotype |
Rats |
Rats, Inbred Strains |
Thymus Gland |
Cytology |
Metabolism |
Ultrastructure |
Celična linija |
Fluorescentna tehnika za protitelesa |
Priželjc |
Protitelesa monoklonska |
Mikroskopija elektronska |
Živali |
Podgane, čiste linije |
Celična adhezija, molekule |
Podgane |
Fenotip |
Epitelij
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Čolić, Miodrag | ![]() |
Vučević, Dragana | ![]() |
Miyaska, Masayuki | ![]() |
Tamatani, Takuya | ![]() |
Pavlović, Miloš, 1966- | 30480 |
Dujić, Aleksandar | ![]() |
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