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  • Uporabnost celic granuloze za normiranje citometričnih meritev : [magistrsko delo]
    Gazić, Barbara
    Background: The quantity of DNA in the nuclei of the cells measured by cytometry is expressed in arbitrary units and graphically presented by histogram. The DNA value of the sample is compared with ... DNA value of normal cells and expressed by the DNA index. The DNA index indicates a relative levelof deviation from normal diploid cells that have, by definition, a DNA index 1. The selection of appropriate reference cells is of crucial importancefor a proper determination of DNA ploidy. In the fluid of the ovarian follicle that has been aspirated for the purposes of IVF, there is an abundant quantity of granulosa cells that are usually rejected after the removal of oocyte. The aim of our study was to find out whether these granulosa cells (GC) could be applied in the standardization of the measurements of DNA ploidy in fresh, not fixated cells by image- and flow-cytometer. We therefore analyzed the cellular content of follicular fluid, its proliferation characteristics and DNA content of the GC. Methods: In the first series we collected all aspirated follicles of one woman into thesame sample (mixed follicles). We analyzed the follicular fluid sediments of 78 women with gonadotropin stimulated menstrual cycle, 10 women with natural
    Type of material - master's thesis
    Publication and manufacture - Ljubljana : [B. Gazić], 2002
    Language - slovenian
    COBISS.SI-ID - 2398228

Library/institution City Acronym For loan Other holdings
Institute of Oncology Ljubljana Ljubljana OILJ outside loan 1 cop.
MF, Central Medical Library, Ljubljana Ljubljana CMK outside loan 1 cop.
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