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Primerjava molekularno-bioloških metod za identifikacijo borelij lymske borelioze : diplomska naloga = Comparison of the molecular-biological methods for identification of Lyme disease borelia : graduation thesis
Šimenc, Janez, 1973-
Borrelia burgdorferi sensu lato je kompleks veř vrst borelij. NajpomembnejǴe vrste so: B. afzelii, B. gari nii in B. burgdorferi sensu stricto. NaǴtete vrste so povzrořiteljice lymske borelioze pri ...řloveku. Glavni namen dela je razviti metodo PCR, ki loři vrste kompleksa B. burgdorferi sensu lato in primerjati specifiřnost metode PCR z metodo elektroforeze restrikcijskih fragmentov v pulzirajořem polju (PFGE). Vrstno specifiřni oligonukleotidi so pomnoÍevali odsek gena za 16S rRNK. Vzorci so bili isti, kot so bili uporabljeni za PFGE. Analizirali smo 58 vzorcev. Vzorci so bili osamljeni v Laboratoriju za lymsko boreliozo, mikrobioloǴkega inǴtituta Medicinske fakultete v Ljubljani. Metoda PFGE je vedno identificirala samo eno vrsto v vzorcu. Metoda PCR je pri 48 vzorcih pokazala řisto kulturo, isto vrsto kot metoda PFGE. Pri 10 vzorcih pa je metoda PCR pokazala meǴano kulturo. Vedno sta bili identificirani dve vrsti. Nismo ugotovili znařilne razlike v specifiřnosti metod PCR in PFGE (P<0,05).
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [J. Šimenc] ; [BF, Enota medoddelčnega študija mikrobiologije], 2000
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