ALL libraries (COBIB.SI union bibliographic/catalogue database)
13.
Določanje molskih mas ▫$\alpha$▫-amilaze, ▫$\beta$▫-glukozidaze, proteaze in lipaze z metodo gelske kromatografije
Heric, Jasmina, 1991-
Gelska kromatografija je vrsta tekočinske kromatografije in je metoda za ločevanje molekul glede na molekulsko maso. Za gelsko filtracijo uporabljamo steklene kolone, napolnjene s stacionarno (trdno) ...fazo z enakomerno velikostjo por. Volumen oz. prostor med kroglicami gela (prazen volumen kolone) zapolnjuje mobilna (tekoča) faza. Namen magistrske naloge je bil s pomočjo gelske kromatografije določiti molske mase encimov ▫$\alpha$▫-amilaze, ▫$\beta$▫-glukozidaze, proteaze in lipaze. Najprej smo določili skupno koncentracijo proteinov v vzorcih ter določili elucijsi profil encimov. S pomočjo označevalcev znanih molekulskih mas (metilenskega modrila in dekstran modrega) smo izrisali umeritveno krivuljo za določanje molskih mas in nato določili molske mase encimov ▫$\alpha$▫-amilaze, ▫$\beta$▫-glukozidaze, proteaze in lipaze. Ugotovili smo, da je eksperimentalno določena molska masa ▫$\alpha$▫-amilaze je 54,6 kDa, ▫$\beta$▫-glukozidaze 112,2 kDa, proteaze 78,3 kDa in lipaze 26,6 kDa. Rezultate eksperimentalno določenih molskih mas smo primerjali s podatki o molskih masah najdenih v literaturi. Nato smo vse štiri encime združili v mešanico 4 encimov, katerim smo prav tako določili skupno koncentracijo proteinov s pomočjo Bradfordove metode in nato določili aktivnost posameznih encimov ▫$\alpha$▫-amilaze, ▫$\beta$▫-glukozidaze, proteaze in lipaze v mešanici 4 encimov.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Maribor : [J. Heric], 2019
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
