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A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeastAndersen, Gorm ...In humans, beta-alanine (BAL) and the neurotransmitter gamma-aminobutyrate (GABA) are transaminated by a single aminotransferase enzyme. Apparently, yeast originally also had a single enzyme, but the ... corresponding gene was duplicated in the Saccharomyces kluyveri lineage. SkUGA1 encodes a homologue of Saccharomyces cerevisiae GABA aminotransferase, and SkPYD4 encodes an enzyme involved in both BAL and GABA transamination. SkPYD4 and SkUGA1 as wellas S. cerevisiae UGA1 and Schizosaccharomyces pombe UGA1 were subcloned, over-expressed and purified. One discontinuous and two continuous coupled assays were used to characterize the substrate specificity and kinetic parameters of the four enzymes. It was found that the cofactor pyridoxal 5'-phosphate is needed for enzymatic activity and alpha-ketoglutarate, and notpyruvate, as the amino group acceptor. SkPyd4p preferentially uses BAL as the amino group donor (V(max)/K(m)=0.78 U x mg(-1) x mm(-1)), but can also useGABA (V(max)/K(m)=0.42 U x mg(-1) x mm(-1)), while SkUga1p only uses GABA (V(max)/K(m)=4.01 U x mg(-1) x mm(-1)). SpUga1p and ScUga1p transaminate only GABA and not BAL. While mammals degrade BAL and GABA with only one enzyme, butin different tissues, S. kluyveri and related yeasts have two different genes/enzymes to apparently 'distinguish' between the two reactions in a single cell. It is likely that upon duplication approximately 200 million years ago, a specialized Uga1p evolved into a 'novel' transaminase enzyme withbroader substrate specificity.Source: FEBS journal. - ISSN 1742-464X (Letn. 274, št. 7, 2007, str. 1804-1817)Type of material - article, component partPublish date - 2007Language - englishCOBISS.SI-ID - 25354969
Author
Andersen, Gorm |
Andersen, Birgit |
Dobritzsch, Doreen |
Schnackerz, Klaus D. |
Piškur, Jure, 1960-2014
Topics
Catalysis |
Cloning, Molecular |
Enzyme Stability |
Fungal Proteins |
Genetics |
Metabolism |
Genotype |
Hydrogen-Ion Concentration |
Kinetics |
Molecular Sequence Data |
Mutation |
Phenotype |
Phylogeny |
Saccharomyces Cerevisiae |
Enzymology |
Genetics |
Metabolism |
Schizosaccharomyces |
Enzymology |
Genetics |
Metabolism |
Spectrum Analysis |
Substrate Specificity |
Yeasts |
Enzymology |
Genetics |
Metabolism |
Beta-Alanine |
Metabolism |
Kloniranje molekulsko |
Filogeneza |
Saccharomyces cerevisiae |
Substrat, specifičnost |
Encim, stabilnost |
Molekulsko zaporedje, podatki |
Genotip |
Kinetika |
Spektralna analiza |
Beta-alanin |
Fenotip |
Kataliza |
pH |
Kvasovke |
Glivične beljakovine |
Mutacija |
Schizosaccharomyces
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Andersen, Gorm | ![]() |
Andersen, Birgit | ![]() |
Dobritzsch, Doreen | ![]() |
Schnackerz, Klaus D. | ![]() |
Piškur, Jure, 1960-2014 | 31275 |
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