-
Rickettsial agents in Slovakian ticks (Acarina, Ixodidae) and their ability togrow in Vero and L929 cell linesBoldiš, Vojtech ...A total of 80 adult ticks (55 Haemaphysalis inerrrzis, 12 Dermacentor rečiculatus, 11 D. marginatus, 2 Ixodes ricinus) were collected from vegetation in three areas of Slovakia (forest and pasture ... habitat) in central Europe. Forty-six (46 ticks) (57.5%) of all species tested were positive by the hemocyte test, PCR assays based on thegltA and ompA genes showed a Rickettsiaceae infection in 77.5% of the ticks, whereas only one H. inermis tick was positive for Anaplasmataceae on a 16S rRNA-based PCR. Isolation of rickettsiae was attempted on all collected ticks by means of the shell vial technique, 52 isolates of which were inoculated into Vero cells and 28 into L929 cells. Rickettsiae were detected in 50% (40/80) of the cell lines using the Gimenez staining method, whereas 33.8% (27/80) of the cell lines were PCR-positive for Rickettsia species. The presence of rickettsiae was shown by PCR to be around 30.8% (16/52) in Vero and 39.3% (11/28) in L929 cell lines. Sequencing results showed that detected infections were Rickettsia sp., R. raoultii, and Anaplasma phagocyčophilum in ticks, and R. slovaca in cell lines. This is the first report of R. raoultii in Slovakia. Observations by electron microscopy of the R, slovaca isolate from Vero cell lines showed a microcapsular layer, typical Gram-negative cell wall, and a cytoplasmic membrane.Source: Annals of the New York Academy of Sciences. - ISSN 0077-8923 (Letn. 1149, 2008, str. 281-285)Type of material - article, component partPublish date - 2008Language - englishCOBISS.SI-ID - 25458905
Author
Boldiš, Vojtech |
Kocianová, Elena |
Štrus, Jasna |
Tušek-Žnidarič, Magda |
Sparagano, Oliver A. |
Štefanidesová, Katarina |
Špitalská, Eva
Topics
Ticks |
Microbiology |
Rickettsia |
Isolation And Purification |
Anaplasmataceae |
Isolation And Purification |
Polymerase Chain Reaction |
Sequence Analysis, Dna |
Vero Cells |
Slovakia |
Polimerazna, verižna reakcija |
Zaporedje, analiza, DNA |
Klopi |
Rickettsia |
Anaplasmataceae |
Vero celice |
Slovaška |
Ixodidae
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Boldiš, Vojtech | ![]() |
Kocianová, Elena | ![]() |
Štrus, Jasna | 07737 |
Tušek-Žnidarič, Magda | 09864 |
Sparagano, Oliver A. | ![]() |
Štefanidesová, Katarina | ![]() |
Špitalská, Eva | ![]() |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.