-
Macroscopic properties of phospholipid vesicles with a contact angle between the membrane domainsBožič, Bojan, 1965- ; Majhenc, JanjaTernary mixtures of a high-melting lipid, a low-melting lipid, and cholesterolare known to form domains of a liquid-ordered and a liquid-disordered phase in bilayer membranes. We prepare giant ... vesicles from asphingomyelin/dioleoylphosphocholine/cholesterol mixture and then examine them using fluorescence microscopy. NBD-labeled lipid and BODIPY-labeled cholesterol are used 2o identify the phase domains of the membrane. A vesicle with only two domains, one in a liquid-ordered and one in a liquid-disordered phase, is chosen because of its simple geometry, for convenient comparison of the experimental results with the theoretical predictions. A microinjector is used to gradually decrease and/or increase the volume of the vesicles by changing the osmolarity of the sugar solution. The relevant energy terms of the membrane mechanics are the elastic energies of the domains and the energy of the domain boundary. The elastic energy of the membrane domains can be described by two terms: the bending energy and the Gaussian bending energy. The energy of the domain boundary is proportional to its length. At the boundary between the domains a contact angle is taken into consideration. Then, in order to obtain values for the lateral tension and the contact angle,the areas of the domains and the characteristic dimensions of the shape are determined for different volumes. The best fits were obtained for a line tension of 6 +/- 3 pN and a contact angle of 1.4 +/- 0.3 rad.Source: ChemPhysChem : a European journal of chemical physics and physical chemistry. - ISSN 1439-4235 (Vol. 10, no. 16, 2009, str. 2862-2870)Type of material - article, component part ; adult, seriousPublish date - 2009Language - englishCOBISS.SI-ID - 26244313
Author
Božič, Bojan, 1965- |
Majhenc, Janja
Topics
Biophysics |
Membranes, Artificial |
Phospholipids |
Membrane Fluidity |
Lipid Bilayers |
Particle Size |
Elasticity |
Microscopy, Fluorescence |
Models, Theoretical |
Fosfolipidi |
Lipidna, dvojna plast |
Biofizika |
Membranska fluidnost |
Membrane umetne |
Delec, velikost |
Elastičnost |
Mikroskopija fluorescenčna |
Modeli teoretski
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Božič, Bojan, 1965- | 11088 |
Majhenc, Janja | 12001 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.