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Anticryptococcal cytotoxicity of murine nonadherent cells is perforin and nonperforin mediatedPetković, Ljubica ...The encapsulated fungal pathogen Cryptococcus neoformans is a significant agent of life-threatening infections, particularly in people with suppressed cell-mediated immunity. The cellular ... cytotoxicity against C. neoformans infection is mainly mediated by NK and T cells, but effector mechanisms are not well understood. The objective of this study was (i) to determine whether prior exposure to the cryptococcal antigens enhances anticryptococcal activityof cytotoxic cells in mice and (ii) the contribution of perforin- and nonperforin-mediated cytotoxicity of NK and T cells in growth inhibition of C.neoformans. Our data showed that in vitro exposure of nonadherent (NA) spleen mononuclear cells from nonimmunized mice to heat-killed C. neoformans strain Cap67 unencapsulated mutant of B3501 (Ag1) or its supernatant (Ag2) demonstrated higher anticryptococcal activity. This effector mechanism can beenhanced further after immunization with either Ag1 or Ag2. There is a synergistic effect of immunization and in vitro incubation of the NA cells with the same antigens. Concanamycin A (CMA) and strontium chloride (SrCl2) inhibition assays were performed to clarify the contribution of perforin- and nonperforin-mediated anticryptococcal cytotoxicity of NA cells in these events. Treatment with these inhibitors demonstrated that anticryptococcal cytotoxicity of nonprimed NA cells was primarily perforin mediated. Anticryptococcal activity of the NA cells obtained from immunized mice after in vitro incubation with cryptococcal antigens was both perforin and nonperforin mediated. Taken together these data demonstrate that in mice a nonperforin-mediated pathway of anticryptococcal cytotoxicity can be induced by immunization. Further research is needed to examine their potential role for human vaccines strategies and/or therapies.Source: Mycologia. - ISSN 0027-5514 (Letn. 102, št. 3, 2010, str. 552-559)Type of material - article, component partPublish date - 2010Language - englishCOBISS.SI-ID - 27334617
Author
Petković, Ljubica |
Arsic Arsenijević, Valentina |
Skvarč, Miha, 1978- |
Kopitar, Andreja Nataša |
Kotnik, Vladimir |
Simčič, Saša |
Wraber-Herzog, Branka |
Matos, Tadeja |
Ihan, Alojz
Topics
Cryptococcosis |
Microbiology |
Immunology |
Immunity, Cellular |
Antigens, Fungal |
Cryptococcus Neoformans |
Growth And Development |
Immunology |
Cytotoxicity, Immunologic |
Immunization |
Leukocytes, Mononuclear |
Cytology |
Immunology |
Mice |
Mice, Inbred Balb C |
Spleen |
Immunology |
T-Lymphocytes |
Immunology |
Concanavalin A |
Diagnostic Use |
Strontium |
Diagnostic Use |
Cryptococcus neoformans |
Citotoksičnost imunska |
Kriptokokoza |
Antigeni glivični |
Imunost celična |
Levkociti mononuklearni |
Miši seva BALB C |
Limfociti T |
Imunizacija |
Vranica |
Stroncij |
Miši |
Konkanavalin A
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Petković, Ljubica | ![]() |
Arsic Arsenijević, Valentina | ![]() |
Skvarč, Miha, 1978- | 27608 |
Kopitar, Andreja Nataša | 25991 |
Kotnik, Vladimir | 07756 |
Simčič, Saša | 05382 |
Wraber-Herzog, Branka | 04890 |
Matos, Tadeja | 18993 |
Ihan, Alojz | 10337 |
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