ALL libraries (COBIB.SI union bibliographic/catalogue database)
13.
Time- and temperature-dependent autolysis of urinary bladder epithelium during ex vivo preservation
Erman, Andreja ; Veranič, Peter
Morphological and functional preservation of urinary bladder epithelium-urothelium after extirpation from an organism enables physiologicalstudies of that tissue and provides the basis for successful ...organ transplantations. The aim of this study was to determine the optimal temperature for maintaining urothelium in ex vivo conditions. Mouse urinary bladders were kept at the three temperatures usually used for maintaining tissue during transportation: at the temperature of melting ice (1 degrees C),at room temperature (22-24 degrees C), and at the body temperature of most mammals (37 degrees C). Autolytic structural changes were followed with electron microscopy, while destruction of cytoskeleton and intercellular junctions was observed by immunolabeling. The first ultrastructural changes, swelling of mitochondria and necrosis of individual cells, became evident 30 min after extirpation if the tissue was kept at 1 degrees C. After 60 and 120 min in ex vivo conditions, the most severe changes with increasing plasma membrane ruptures were detected at 1 degrees C, while at room temperature onlymild changes were detected. At 37 degrees C, the extent of ultrastructuralchanges was between those of the other two experimental temperatures. Autolytic destruction of cytoskeleton and intercellular junctions was not observed before 2 h after extirpation. After 4 h, severe degradation of cytokeratin 20 and microtubules were found at 1 degrees C and 37 degrees C, while being almost undisturbed at room temperature. On the otherhand, the reduction of desmoplakin and ZO-1 labeling was more evident at 37 degrees C than at 1 degrees C and room temperature. These findings provide evidence that room temperature is most appropriate for short ex vivo preservation of urothelial tissue.
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
