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In vitro plant regeneration from Pepper (Capsicum annuum L. cv. 'Soroksari') seedling explants
Berljak, Jasna
Adventitous and axillary buds, shoots and needles were generated using tissue cultures from excised embryos of Pinus nigra Arnold. Formation of these structures in embryo culture was obtained on agar ...half-strength MS basal medium supplemented with 2 mg. 1-1 BAP, 250 mg. 1-1 myo-inositol and NAA and IBA in various concentrations. Using shoots and needles was also achieved on an agar medium of half-strenght MS salts, but supplemented with 1 mg. 1-1 BAP and 0.001 mg. 1-1 NAA. The origins of new buds in these cultures were adventitious, axillary and native form brachyblasts. Long-shoot development and growth of buds and shoots was achieved development and growth of buds and shoots was achieved by transferring them into a hormone-free medium, sometimesaided by the addition of 0.3% activated charcoal. The best tissue-media interactions produced bud and shoot formation in 60-70% of cultures. In-vitro developed shoots were subjected to the same procedures, andtypically produced a successive generation of buds, shoots and needles.
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