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Golgi apparatus fragmentation as a mechanism responsible for uniform delivery of uroplakins to the apical plasma membrane of uroepithelial cellsErdani-Kreft, Mateja ...Background information. The GA (Golgi apparatus) has an essential role in membrane trafficking, determining the assembly and delivery of UPs (uroplakins) to the APM (apical plasma membrane) of ... superficial UCs (uroepithelial cells) of urinary bladder. UPs are synchronously and uniformly delivered from the GA to the APM by DFVs (discoidalor fusiform-shaped vesicles); however, themechanism of UP delivery is not known.We have used the culture model of UCs with the capacity to undergo terminal differentiation to study the process of uniform delivery of DFVs to the APM and to elucidate the mechanisms involved. Results. By three-dimensional localization using confocalmicroscopy of immunofluorescence-labelled GA-related markers šGM130 (cis-Golgi matrix protein of 130 kDa), GS15 (Golgi Snare 15 kDa), GS28 and giantinđ, uroepithelial differentiation-related markers (UPs), MTs (microtubules; á-tubulin) and intermediate filaments šCK7 (cytokeratin 7) and CK20đ, we found that in non-differentiated, UP-negative UCs the GA is mostly organized as a single ribbonlike structure close to the nucleus, whereas in differentiated, UP-positive UCs the GA is fragmented and spread almost throughthe entire cell. The FRAP (fluorescence recovery after photobleaching) experiments on the UCs transfected with GalT (trans-Golgi/TGN enzyme â1,4-galactosyltransferase) fused to fluorescent protein showed that Golgi-resident enzyme cycles freely within ribbon-like GA but not within fragmented GA. By CLEM (correlative light-electron microscopy), we examined the GA fragments in cells expressing UPs. We found that GA fragments are fullyfunctional and similar to the GA fragments that are formed after nocodazole treatment. (Abstract truncated at 2000 characters)Source: Biology of the Cell. - ISSN 0248-4900 (Letn. 102, št. 11, 2010, str. 593-607)Type of material - article, component partPublish date - 2010Language - englishCOBISS.SI-ID - 27453657
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Database name | Field | Year |
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Erdani-Kreft, Mateja | 15873 |
Giandomenico, Daniele, Di | |
Beznoussenko, Galina V | |
Resnik, Nataša | 28393 |
Mironov, Alexander A. | |
Jezernik, Kristijan | 05277 |
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