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Identification and characterization of putative osmosensors, HwSho1A and HwSho1B, from the extremely halotolerant black yeast Hortaea werneckiiFettich, Martin ...In Saccharomyces cerevisiae, the Sho1 protein is one of two potential osmosensors that can activate the kinase cascade of the HOG pathway in response to increased extracellular osmolarity. Two novel ... SHO1-like genes, HwSHO1A and HwSHO1B, have been cloned from the saltern-inhabiting, extremely halotolerant black yeast Hortaea werneckii. The HwSho1 protein isoforms are 93.8% identical in their amino-acid sequences, and have a conserved SH3 domain. When the HwSHO1 genes were transferred into S. cerevisae cells lackingthe SHO1 gene, both of the HwSho1 isoforms fully complemented the function of the native S. cerevisiae Sho1 protein. Through microscopic and biochemical validation, we demonstrate that in S. cerevisiae, both of the HwSho1 proteins have characteristic subcellular localizations similar to the S. cerevisiae Sho1 protein, and they can both activate the HOG pathway under conditions of osmotic stress. To a lower extent, crosstalk to the mating pathway expressing HwSho1 proteins is conserved in the PBS2 deleted S. cerevisiae strain. These data show that the HwSho1 proteins from H. werneckiiare true functional homologs of the Sho1 protein of S. cerevisiae.Source: Fungal genetics and biology. - ISSN 1087-1845 (Vol. 48, issue 5, 2011, str. 475-484)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 28077785
Author
Fettich, Martin |
Lenassi, Metka, 1978- |
Veranič, Peter |
Gunde-Cimerman, Nina |
Plemenitaš, Ana, 1952-
Topics
Yeasts |
Osmolar Concentration |
Sodium Chloride |
Receptors, Sensory |
Signal Transduction |
Glycerin |
Polymerase Chain Reaction |
Beta-Galactosidase |
Blotting, Western |
Microscopy, Fluorescence |
Phylogeny |
Receptorji senzorični |
Signal, prevajanje |
Osmolarna koncentracija |
Natrijev klorid |
Kvasovke |
Polimerazna, verižna reakcija |
Filogeneza |
Beta-galaktozidaza |
Blot, Western |
Mikroskopija fluorescenčna
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Fettich, Martin | 29348 |
Lenassi, Metka, 1978- | 24288 |
Veranič, Peter | 08279 |
Gunde-Cimerman, Nina | 05935 |
Plemenitaš, Ana, 1952- | 06777 |
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