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The impact of sleep deprivation and nighttime light exposure on clock gene expression in humansKavčič, Pavel, 1980- ...Aim To examine the effect of acute sleep deprivation under light conditions onthe expression of two key clock genes, hPer2 and hBmal1, in peripheral bloodmononuclear cells (PBMC) and on plasma ... melatonin and cortisol levels. Methods Blood samples were drawn from 6 healthy individuals at 4-hour intervals for three consecutive nights, including a night of total sleep deprivation (second night). The study was conducted in April-June 2006 at the UniversityMedical Centre Ljubljana. Results We found a significant diurnal variation in hPer2 and hBmal1 expression levels under baseline (P < 0.001, F =19.7, df = 30 for hPer2 and P < 0.001, F = 17.6, df = 30 for hBmal1) and sleep-deprived conditions (P < 0.001, F = 9.2, df = 30 for hPer2 and P < 0.001, F = 13.2, df = 30 for hBmal1). Statistical analysis with the single cosinor method revealed circadian variation of hPer2 under baseline and of hBmal1 under baseline and sleep-deprived conditions. The peak expression of hPer2 was at 13:55 1:15 hours under baseline conditions and of hBmal1 at 16:08 1:18 hours under baseline and at 17:13 1:35 hours under sleep-deprived conditions. Individual cosinor analysis of hPer2 revealed a loss of circadian rhythm in 3 participants and a phase shift in 2 participantsunder sleep-deprived conditions. The plasma melatonin and cortisolrhythms confirmed a conventional alignment of the central circadian pacemaker to the habitual sleep/wake schedule. Conclusion Our results suggest that 40-hour acute sleep deprivation under light conditions may affect the expression of hPer2 in PBMCs.Source: Croatian medical journal. - ISSN 0353-9504 (Vol. 52, 2011, str. 619-628)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 28925657
Author
Kavčič, Pavel, 1980- |
Rojc, Bojan, dr. med., 1980- |
Dolenc-Grošelj, Leja |
Claustrat, Bruno |
Fujs Komloš, Kristina |
Poljak, Mario, 1965-
Topics
Sleep Deprivation |
Light |
Biological Clocks |
Gene Expression |
Monocytes |
Melatonin |
Blood |
Hydrocortisone |
Rna, Messenger |
Polymerase Chain Reaction |
Circadian Rhythm |
Spanje, deprivacija |
Gensko izražanje |
Svetloba |
Monociti |
Biološke ure |
RNA, prenašalna |
Polimerazna, verižna reakcija |
Hidrokortizon |
Dnevni ritem |
Melatonin
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Kavčič, Pavel, 1980- | |
Rojc, Bojan, dr. med., 1980- | 33913 |
Dolenc-Grošelj, Leja | 15400 |
Claustrat, Bruno | |
Fujs Komloš, Kristina | 25986 |
Poljak, Mario, 1965- | 11622 |
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