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  • Colocalization of serum amyloid A with microtubules in human coronary artery endothelial cells [Elektronski vir]
    Lakota, Katja ...
    Serum amyloid A (SAA) acts as a major acute phase protein and represents a sensitive and accurate marker of inflammation. Besides its hepatic origin, as the main source of serum SAA, this protein is ... also produced extrahepatically. The mRNA levels of SAA become significantly elevated following proinflammatorystimuli, as well as, are induced through their own positive feedback in human primary coronary artery endothelial cells. However, the intracellular functions of SAA are so far unknown. Colocalization of SAA with cytoskeletal filaments has previously been proposed, so we analyzed the colocalization of SAA with all three cytoskeletal elements: actin filaments, vimentin filaments, and microtubules. Immunofluorescent double-labeling analyses confirmed by PLA method revealed a strict colocalization of SAA with microtubules and a very infrequent attachment to vimentin while the distribution of actin filaments appeared clearly separated from SAA staining. Also, no significant colocalization was found between SAA and endomembranes labeled with the fluorescent lipid stain DiO6. However, SAA appears to be located also unbound in the cytosol, as well as inside the nucleus and within nanotubes extending from the cells or bridging neighboring cells. These different locations of SAA in endothelial cells strongly indicate multiple potential functions of this protein.
    Source: Journal of Biomedicine and Biotechnology (Online) [Elektronski vir]. - ISSN 1110-7251 (2011, Str. [1-8], 528276)
    Type of material - e-article
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 29065689

    Link(s):

    http://www.hindawi.com/journals/jbb/2011/528276
    DOI

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