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Process optimization and biocompatibility of cell carriers suitable for automated magnetic manipulationKrejci, I. ...There is increasing demand for automated cell reprogramming in the fields of cell biology, biotechnology and the biomedical sciences. Microfluidic-based platforms that provide unattended manipulation ... of adherent cells promise to be an appropriate basis for cell manipulation. In this study we developed a magnetically driven cell carrier to serve as a vehicle within an in vitro environment. To elucidate the impact of the carrier on cells, biocompatibilitywas estimated using the human adenocarcinoma cell line Caco-2.Besides evaluation of the quality of the magnetic carriers by field emission scanning electron microscopy, the rate of adherence, proliferation and differentiation of Caco-2 cells grown on the carriers was quantified. Moreover, the morphology of the cells was monitored by immunofluorescent staining. Early generations of the cell carrier suffered from release of cytotoxic nickel from the magnetic cushion. Biocompatibility was achieved by complete encapsulation of the nickel bulk within galvanic gold. The insulationprocess had to be developed stepwise and was controlled by parallel monitoring of the cell viability. The final carrier generation proved to be a proper support for cell manipulation, allowing proliferation of Caco-2 cells equal to that on glass or polystyrene as a reference for up to 10days. Functional differentiation was enhanced by more than 30% compared with the reference. A flat, ferromagnetic and fully biocompatible carrier for cell manipulation was developed for application in microfluidic systems. Beyond that, this study offers advice for the development of magnetic cell carriers and the estimation of their biocompatibility.Source: Acta biomaterialia. - ISSN 1742-7061 (Vol. 8, issue 3, 2012, str. 1239-1247)Type of material - article, component partPublish date - 2012Language - englishCOBISS.SI-ID - 29523929
Author
Krejci, I. |
Piana, C. |
Wegener, T. |
Batista, Urška |
Zemljič Jokhadar, Špela
Topics
Caco-2 Cells |
Biotechnology |
Carrier Proteins |
Vehicles |
Biocompatible Materials |
Nickel |
Electromagnetic Fields |
Culture Media |
Glass |
Polystyrenes |
Cell Survival |
Cell Adhesion |
Microscopy, Electron, Scanning |
Biokompatibilni materiali |
Biotehnologija |
Zdravilo, podlage |
Nosilne beljakovine |
Caco-2 celice |
Polistireni |
Celica, preživetje |
Celična adhezija |
Mikroskopija elektronska, vrstična |
Gojišče |
Nikelj |
Elektromagnetna polja |
Steklo
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Krejci, I. | ![]() |
Piana, C. | ![]() |
Wegener, T. | ![]() |
Batista, Urška | 08588 |
Zemljič Jokhadar, Špela | 26234 |
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